PFKL Antibody – Rabbit Polyclonal

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq, Aug 2008]

PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - galactose; Kinase, other; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: 6-phosphofructokinase complex; membrane; cytosol

Molecular Function: identical protein binding; protein binding; metal ion binding; kinase binding; ATP binding; 6-phosphofructokinase activity

Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; response to glucose stimulus; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process; protein oligomerization; protein homotetramerization; negative regulation of insulin secretion