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PAF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904562

PCNA associated factor (PAF) is a PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion… More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PAF, Polyclonal Antibody
Full Product Name
Rabbit anti-PAF Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
PCLAF
Product Synonym Names
HCV NS5A-transactivated protein 9; PCNA-clamp-associated factor; PCNA-associated factor of 15 kDa; p15(PAF); PCNA-associated factor; PAF15; PAF; p15PAF; p15/PAF; of 15 kDa; OEATC-1; OEATC1; OEATC; NS5ATP9; L5; hepatitis C virus NS5A-transactivated protein 9; KIAA0101; overexpressed in anaplastic thyroid carcinoma 1; PCNA-associated factor; PCNA clamp associated factor
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
7,221 Da
OMIM
610696
3D Structure
ModBase 3D Structure for Q15004
Immunogen
Between 61 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-PAF antibody

Applications Tested/Suitable for anti-PAF antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PAF antibody
WB: 1:2000-1:10,000
IHC: 1:2000-1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-PAF antibody

Detection of human PAF by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PAF (Cat. No. MBS3904562 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Rabbit Anti-PAF Polyclonal Antibody – Cat# MBS3904562

Western Blot (WB) of anti-PAF antibody

Detection of human PAF by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PAF antibody MBS3904562 (lot MBS3904562-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-PAF Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904562

Other Notes

Small volumes of anti-PAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAF purchase

MBS3904562 is an antibody targeting PAF. Also known as Rabbit anti-PAF Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
PCNA-associated factor isoform 1
NCBI Official Synonym Full Names
KIAA0101
NCBI Official Symbol
KIAA0101
NCBI Official Synonym Symbols
L5; PAF; OEATC; PAF15; OEATC1; p15PAF; NS5ATP9; OEATC-1; p15/PAF; p15(PAF)
UniProt Protein Name
PCNA-associated factor
UniProt Synonym Protein Names
Hepatitis C virus NS5A-transactivated protein 9; HCV NS5A-transactivated protein 9; Overexpressed in anaplastic thyroid carcinoma 1; OEATC-1; PCNA-associated factor of 15 kDa; PAF15; p15PAF
UniProt Gene Name
KIAA0101
UniProt Synonym Gene Names
NS5ATP9; PAF; HCV NS5A-transactivated protein 9; OEATC-1; PAF15; p15PAF
UniProt Entry Name
PAF15_HUMAN
NCBI Protein Information
PCNA-associated factor

UniProt Comments for PAF

PAF: May be involved in protection of cells from UV-induced cell death.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: bypass DNA synthesis; centrosome organization and biogenesis; regulation of cell cycle; DNA replication; response to DNA damage stimulus; response to UV
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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