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Myoferlin (MYOF) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904575

Myoferlin is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Myoferlin is also Involved in endocytic recycling and… More details

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Product Overview

Online Datasheet in PDF
Product Name
Myoferlin, Polyclonal Antibody
Full Product Name
Rabbit anti-Myoferlin Antibody, Affinity Purified
Product Gene Name
anti-MYOF antibody
Product Synonym Names
fer-1-like protein 3; myoferlin; fer-1-like family member 3; fer-1-like 3, myoferlin; FER1L3; Myoferlin; myoferlin
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
604603
3D Structure
ModBase 3D Structure for Q9NZM1
Immunogen
Between 700 and 750
Conjugation
Unconjugated

Product Categories/Family for anti-MYOF antibody

Primary Antibodies
Applications Tested/Suitable for anti-MYOF antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MYOF antibody
WB: 1:1000-1:5000 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-MYOF antibody

Detection of human Myoferlin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Myoferlin antibody MBS3904575 (lot MBS3904575-1) used for IP at 6 ug per reaction. Myoferlin was also immunoprecipitated by rabbit anti-Myoferlin antibody BL16508. For blotting immunoprecipitated Myoferlin, MBS3904575 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-MYOF Polyclonal Antibody – Cat# MBS3904575

Western Blot (WB) of anti-MYOF antibody

Detection of human Myoferlin by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Myoferlin antibody MBS3904575 (lot MBS3904575-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-MYOF Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904575

Other Notes

Small volumes of anti-MYOF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYOF purchase

MBS3904575 is an antibody targeting MYOF. Also known as Rabbit anti-Myoferlin Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MYOF. It may not necessarily be applicable to this product.

NCBI GeneID
26509
NCBI Accession #
NP_579899.1
UniProt Primary Accession #
Q9NZM1
UniProt Protein Name
Myoferlin
UniProt Synonym Protein Names
Fer-1-like protein 3
Protein Family
Myoferlin
UniProt Gene Name
MYOF
UniProt Synonym Gene Names
FER1L3; KIAA1207
UniProt Entry Name
MYOF_HUMAN

UniProt Comments for MYOF

myoferlin: Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR. Belongs to the ferlin family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nuclear membrane; cytoplasmic vesicle membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; nuclear envelope; caveola; cytoplasmic vesicle

Molecular Function: protein binding; phospholipid binding

Biological Process: muscle contraction; plasma membrane repair; blood circulation; regulation of vascular endothelial growth factor receptor signaling pathway; myoblast fusion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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