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AE2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904676

Anion exchange protein 2 (AE2) is a plasma membrane anion exchange protein of wide distribution [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P04920]. More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
AE2, Polyclonal Antibody
Full Product Name
Rabbit anti-AE2 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
SLC4A2
Product Synonym Names
anion exchanger 2 type b2; solute carrier family 4 (anion exchanger), member 2; non-erythroid band 3-like protein; NBND3; HKB3; erythrocyte membrane protein band 3-like 1; Solute carrier family 4 member 2; anion exchanger 2 type b1; anion exchanger 2 type a; Anion exchanger 2; anion exchange protein 2; AE2; AE 2; EPB3L1; BND3L; Anion exchange protein 2; solute carrier family 4 member 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
137,009 Da
OMIM
109280
3D Structure
ModBase 3D Structure for P04920
Predicted Reactivity
Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-AE2 antibody

Applications Tested/Suitable for anti-AE2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-AE2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-AE2 antibody

Detection of human AE2 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AE2 (Cat. No. MBS3904676 Lot1, left image and Cat. No. MBS3904677 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-AE2 Polyclonal Antibody – Cat# MBS3904676

Immunoprecipitation (IP) of anti-AE2 antibody

Detection of human AE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody MBS3904676 (lot MBS3904676-1) used for IP at 6 ug per reaction. AE2 was also immunoprecipitated by rabbit anti-AE2 antibody MBS3904677. For blotting immunoprecipitated AE2, MBS3904677 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-AE2 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904676

Western Blot (WB) of anti-AE2 antibody

Detection of human AE2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-AE2 antibody MBS3904676 (lot MBS3904676-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-AE2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904676

Other Notes

Small volumes of anti-AE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AE2 purchase

MBS3904676 is an antibody targeting AE2. Also known as Rabbit anti-AE2 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AE2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
anion exchange protein 2 isoform 1
NCBI Official Synonym Full Names
solute carrier family 4 (anion exchanger), member 2
NCBI Official Symbol
SLC4A2
NCBI Official Synonym Symbols
AE2; HKB3; BND3L; NBND3; EPB3L1
UniProt Protein Name
Anion exchange protein 2
UniProt Synonym Protein Names
Non-erythroid band 3-like protein; BND3L; Solute carrier family 4 member 2
Protein Family
UniProt Gene Name
SLC4A2
UniProt Synonym Gene Names
AE2; EPB3L1; HKB3; MPB3L; AE 2; Anion exchanger 2; BND3L
UniProt Entry Name
B3A2_HUMAN
NCBI Protein Information
anion exchange protein 2; AE 2; anion exchanger 2 type a; anion exchanger 2 type b1; anion exchanger 2 type b2; solute carrier family 4 member 2; non-erythroid band 3-like protein; erythrocyte membrane protein band 3-like 1

UniProt Comments for AE2

SLC4A2: Plasma membrane anion exchange protein of wide distribution. Belongs to the anion exchanger (TC 2.A.31) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: focal adhesion; membrane; basolateral plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: chloride transmembrane transporter activity; inorganic anion exchanger activity; anion transmembrane transporter activity

Biological Process: bicarbonate transport; regulation of intracellular pH; ion transport; transmembrane transport; anion transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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