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COPB2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904694

In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known… More details

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Product Overview

Online Datasheet in PDF
Product Name
COPB2, Polyclonal Antibody
Full Product Name
Rabbit anti-COPB2 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
p102; beta'-COP; betaprime-COP; coatomer binding complex, beta prime subunit; coatomer protein complex subunit beta prime; coatomer protein complex, subunit beta 2 (beta prime); coatomer subunit beta'; MCPH19; beta'-coat protein; Coatomer subunit beta'; coatomer protein complex subunit beta 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
Predicted MW: 105.919kDa
3D Structure
ModBase 3D Structure for P35606
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 856 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-COPB2 antibody

Applications Tested/Suitable for anti-COPB2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-COPB2 antibody
WB: 1:10,000-1:25,000
IP: 6ug/mg lysate
IHC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-COPB2 antibody

Detection of human COPB2 by immunohistochemistry. Samples: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-COPB2 (MBS3904694 Lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Rabbit Anti-COPB2 Polyclonal Antibody – Cat# MBS3904694

Immunohistochemistry (IHC) of anti-COPB2 antibody

Detection of mouse COPB2 by immunohistochemistry. Samples: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-COPB2 (MBS3904694 Lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-COPB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3904694

Immunoprecipitation (IP) of anti-COPB2 antibody

Detection of human COPB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB2 antibody (MBS3904694 lot 2) used for IP at 6 ug per reaction. COPB2 was also immunoprecipitated by a previous lot of this antibody (MBS3904694 lot 1) and a second antibody against a different epitope of COPB2 (MBS3904693). For blotting immunoprecipitated COPB2, MBS3904694 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Rabbit Anti-COPB2 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904694

Western Blot (WB) of anti-COPB2 antibody

Detection of mouse COPB2 by western blot. Samples: Whole cell lysate (5 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COPB2 antibody (MBS3904694 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Rabbit Anti-COPB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904694

Western Blot (WB) of anti-COPB2 antibody

Detection of human COPB2 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, HeLa, U2OS, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COPB2 antibody (MBS3904694 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Rabbit Anti-COPB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904694

Other Notes

Small volumes of anti-COPB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COPB2 purchase

MBS3904694 is an antibody targeting COPB2. Also known as Rabbit anti-COPB2 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for COPB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
coatomer subunit beta'
NCBI Official Synonym Full Names
coatomer protein complex subunit beta 2
NCBI Official Symbol
COPB2
NCBI Official Synonym Symbols
MCPH19; beta'-COP
UniProt Protein Name
Coatomer subunit beta'
UniProt Synonym Protein Names
Beta'-coat protein; Beta'-COP; p102
UniProt Gene Name
COPB2
UniProt Synonym Gene Names
Beta'-COP
NCBI Protein Information
coatomer subunit beta'

NCBI Summary for COPB2

The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.[supplied by OMIM, Jul 2002]

UniProt Comments for COPB2

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-COPB2 antibody

 Reference Product  PubMed Publications
 COPA antibody  >1 publications with COPB2 and COPA
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