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Epsin 1 (EPN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904695

Epsin 1 binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). It modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Epsin 1 regulates receptor-mediated… More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Epsin 1 (EPN1), Polyclonal Antibody
Full Product Name
Rabbit anti-Epsin 1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
EH domain-binding mitotic phosphoprotein; EPS-15-interacting protein 1; epsin-1; Epsin-1; epsin 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
3D Structure
ModBase 3D Structure for Q9Y6I3
Predicted Reactivity
Mouse, Rat
Immunogen
Between 375 and 425
Conjugation
Unconjugated

Product Categories/Family for anti-EPN1 antibody

Applications Tested/Suitable for anti-EPN1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-EPN1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-EPN1 antibody

Detection of human Epsin 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Epsin 1 antibody MBS3904695 (lot MBS3904695-1) used for IP at 6 ug per reaction. Epsin 1 was also immunoprecipitated by rabbit anti-Epsin 1 antibody MBS3904696. For blotting immunoprecipitated Epsin 1, MBS3904695 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-EPN1 Polyclonal Antibody – Cat# MBS3904695

Western Blot (WB) of anti-EPN1 antibody

Detection of human and mouse Epsin 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Epsin 1 antibody MBS3904695 (lot MBS3904695-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-EPN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904695

Other Notes

Small volumes of anti-EPN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPN1 purchase

MBS3904695 is an antibody targeting EPN1. Also known as Rabbit anti-Epsin 1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EPN1. It may not necessarily be applicable to this product.

NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Protein Name
Epsin-1
UniProt Synonym Protein Names
EH domain-binding mitotic phosphoprotein; EPS-15-interacting protein 1
Protein Family
UniProt Gene Name
EPN1

UniProt Comments for EPN1

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (). Regulates receptor-mediated endocytosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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