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DYNC1I2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904700

Cytoplasmic dynein 1 intermediate chain 2 (DYNC1I2) acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DYNC1I2, Polyclonal Antibody
Full Product Name
Rabbit anti-DYNC1I2 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
dynein intermediate chain 2, cytosolic; IC2; testis tissue sperm-binding protein Li 66n; dynein, cytoplasmic, intermediate polypeptide 2; DIC74; DH IC-2; cytoplasmic dynein 1 intermediate chain 2; Cytoplasmic dynein intermediate chain 2; DNCI2; Cytoplasmic dynein 1 intermediate chain 2; dynein cytoplasmic 1 intermediate chain 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
603331
3D Structure
ModBase 3D Structure for Q13409
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 588 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-DYNC1I2 antibody

Applications Tested/Suitable for anti-DYNC1I2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-DYNC1I2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-DYNC1I2 antibody

Detection of mouse DYNC1I2 by immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Antibody: Affinity purified rabbit anti-DYNC1I2 (Cat. No. MBS3904699 Lot1, left image and Cat. No. MBS3904700 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-DYNC1I2 Polyclonal Antibody – Cat# MBS3904700

Immunohistochemistry (IHC) of anti-DYNC1I2 antibody

Detection of human DYNC1I2 by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-DYNC1I2 (Cat. No. MBS3904699 Lot1, left image and Cat. No. MBS3904700 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-DYNC1I2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3904700

Immunoprecipitation (IP) of anti-DYNC1I2 antibody

Detection of human DYNC1I2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DYNC1I2 antibody MBS3904700 (lot MBS3904700-1) used for IP at 6 ug per reaction. DYNC1I2 was also immunoprecipitated by rabbit anti-DYNC1I2 antibody MBS3904699. For blotting immunoprecipitated DYNC1I2, MBS3904700 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-DYNC1I2 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904700

Western Blot (WB) of anti-DYNC1I2 antibody

Detection of human and mouse DYNC1I2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DYNC1I2 antibody MBS3904700 (lot MBS3904700-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-DYNC1I2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904700

Other Notes

Small volumes of anti-DYNC1I2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DYNC1I2 purchase

MBS3904700 is an antibody targeting DYNC1I2. Also known as Rabbit anti-DYNC1I2 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DYNC1I2. It may not necessarily be applicable to this product.

NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Protein Name
Cytoplasmic dynein 1 intermediate chain 2
UniProt Synonym Protein Names
Cytoplasmic dynein intermediate chain 2; Dynein intermediate chain 2, cytosolic; DH IC-2
Protein Family
UniProt Gene Name
DYNC1I2
UniProt Synonym Gene Names
DNCI2; DNCIC2; DH IC-2
UniProt Entry Name
DC1I2_HUMAN

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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