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GRASP55 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904702

Golgi reassembly-stacking protein 2 (GRASP55) plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. GRASP55 may regulate the intracellular transport and presentation… More details

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Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GRASP55, Polyclonal Antibody
Full Product Name
Rabbit anti-GRASP55 Antibody, Affinity Purified
Product Gene Name
anti-GRASP55 antibody
Product Synonym Gene Name
GORASP2
Product Synonym Names
p59; GRS2; GOLPH6; GRASP55; Golgi reassembly-stacking protein 2; golgi phosphoprotein 6; golgi reassembly stacking protein 2, 55kDa; Golgi reassembly-stacking protein of 55 kDa; Golgi reassembly-stacking protein 2; golgi reassembly stacking protein 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
608693
3D Structure
ModBase 3D Structure for Q9H8Y8
Immunogen
Between 402 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-GRASP55 antibody

Primary Antibodies
Applications Tested/Suitable for anti-GRASP55 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-GRASP55 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-GRASP55 antibody

Detection of human GRASP55 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GRASP55 (Cat. No. MBS3904702 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-GRASP55 Polyclonal Antibody – Cat# MBS3904702

Immunoprecipitation (IP) of anti-GRASP55 antibody

Detection of human GRASP55 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP55 antibody MBS3904702 (lot MBS3904702-1) used for IP at 6 ug per reaction. GRASP55 was also immunoprecipitated by rabbit anti-GRASP55 antibody BL17153. For blotting immunoprecipitated GRASP55, MBS3904702 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-GRASP55 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904702

Western Blot (WB) of anti-GRASP55 antibody

Detection of human GRASP55 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP55 antibody MBS3904702 (lot MBS3904702-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-GRASP55 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904702

Other Notes

Small volumes of anti-GRASP55 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRASP55 purchase

MBS3904702 is an antibody targeting GRASP55. Also known as Rabbit anti-GRASP55 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GRASP55. It may not necessarily be applicable to this product.

NCBI GeneID
26003
NCBI Accession #
NP_056345.3
UniProt Primary Accession #
Q9H8Y8
UniProt Protein Name
Golgi reassembly-stacking protein 2
UniProt Synonym Protein Names
Golgi phosphoprotein 6; GOLPH6; Golgi reassembly-stacking protein of 55 kDa; GRASP55; p59
Protein Family
Golgi reassembly-stacking protein
UniProt Gene Name
GORASP2
UniProt Synonym Gene Names
GOLPH6; GRS2; GOLPH6; GRASP55
UniProt Entry Name
GORS2_HUMAN

UniProt Comments for GRASP55

GRASP55: a golgi reassembly-stacking protein (GRASP) protein involved in the stacking of Golgi cisternae. May be involved in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as the TGF-alpha receptor. Oligomerization of its N-terminal GRASP domain regulates the formation of Golgi stacks, a process that is regulated by phosphorylation within the C-terminal Ser/Pro-rich domain. Forms a RAB2 effector complex with BLZF1 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of the TGF-alpha receptor, prior its transit in the trans-Golgi. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: Golgi membrane; Golgi apparatus; membrane

Molecular Function: protein binding

Biological Process: organelle organization and biogenesis; mitotic cell cycle
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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