• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GRASP65 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904733

Golgi reassembly-stacking protein of 65 kDa (GRASP65) is a stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. GRASP65 is a key structural protein required for the maintenance of the Golgi apparatus integrity:… More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GRASP65, Polyclonal Antibody
Full Product Name
Rabbit anti-GRASP65 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
GORASP1
Product Synonym Names
golgi reassembly-stacking protein of 65 kDa; P65; GOLPH5; Golgi reassembly-stacking protein 1; golgi reassembly stacking protein 1, 65kDa; Golgi reassembly and stacking protein 1; Golgi phosphoprotein 5; Golgi peripheral membrane protein p65; GRASP65; Golgi reassembly-stacking protein 1; golgi reassembly stacking protein 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
26,237 Da
OMIM
606867
3D Structure
ModBase 3D Structure for Q9BQQ3
Immunogen
Between 390 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-GRASP65 antibody

Applications Tested/Suitable for anti-GRASP65 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-GRASP65 antibody
WB: 1:1000-1:5000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-GRASP65 antibody

Detection of human GRASP65 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody MBS3904733 (lot MBS3904733-1) used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody MBS3904732. For blotting immunoprecipitated GRASP65, MBS3904733 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-GRASP65 Polyclonal Antibody – Cat# MBS3904733

Western Blot (WB) of anti-GRASP65 antibody

Detection of human and mouse GRASP65 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody MBS3904733 (lot MBS3904733-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-GRASP65 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904733

Other Notes

Small volumes of anti-GRASP65 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRASP65 purchase

MBS3904733 is an antibody targeting GRASP65. Also known as Rabbit anti-GRASP65 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GRASP65. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Golgi reassembly-stacking protein 1 isoform 1
NCBI Official Synonym Full Names
golgi reassembly stacking protein 1, 65kDa
NCBI Official Symbol
GORASP1
NCBI Official Synonym Symbols
P65; GOLPH5; GRASP65
UniProt Protein Name
Golgi reassembly-stacking protein 1
UniProt Synonym Protein Names
Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; GOLPH5; Golgi reassembly-stacking protein of 65 kDa; GRASP65
UniProt Gene Name
GORASP1
UniProt Synonym Gene Names
GOLPH5; GRASP65; GOLPH5; GRASP65
UniProt Entry Name
GORS1_HUMAN
NCBI Protein Information
Golgi reassembly-stacking protein 1; Golgi phosphoprotein 5; Golgi peripheral membrane protein p65; Golgi reassembly and stacking protein 1; golgi reassembly-stacking protein of 65 kDa

UniProt Comments for GRASP65

GRASP65: Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes. Belongs to the GORASP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 3p22-p21.33

Cellular Component: Golgi membrane; Golgi apparatus

Biological Process: protein transport; negative regulation of dendrite morphogenesis; mitotic cell cycle; Golgi organization and biogenesis
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-GRASP65 antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000