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NSFL1C Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904735

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of NSFL1C binds a hexamer of cytosolic p97 and is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NSFL1C, Polyclonal Antibody
Full Product Name
Rabbit anti-NSFL1C Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
SHP1 homolog; UBXN2C; UBXD10; UBX domain-containing protein 2C; p97 cofactor p47; P47; NSFL1 cofactor p47; NSFL1 (p97) cofactor (p47); dJ776F14.1; UBX1; NSFL1 cofactor p47; NSFL1 cofactor
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
606610
3D Structure
ModBase 3D Structure for Q9UNZ2
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 50 and 100
Conjugation
Unconjugated

Product Categories/Family for anti-NSFL1C antibody

Applications Tested/Suitable for anti-NSFL1C antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-NSFL1C antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-NSFL1C antibody

Detection of human NSFL1C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody MBS3904735 (lot MBS3904735-1) used for IP at 6 ug per reaction. NSFL1C was also immunoprecipitated by rabbit anti-NSFL1C antibody BL17206. For blotting immunoprecipitated NSFL1C, MBS3904735 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-NSFL1C Polyclonal Antibody – Cat# MBS3904735

Western Blot (WB) of anti-NSFL1C antibody

Detection of human and mouse NSFL1C by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody MBS3904735 (lot MBS3904735-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-NSFL1C Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904735

Other Notes

Small volumes of anti-NSFL1C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NSFL1C purchase

MBS3904735 is an antibody targeting NSFL1C. Also known as Rabbit anti-NSFL1C Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NSFL1C. It may not necessarily be applicable to this product.

NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Protein Name
NSFL1 cofactor p47
UniProt Synonym Protein Names
UBX domain-containing protein 2C; p97 cofactor p47
Protein Family
UniProt Gene Name
NSFL1C
UniProt Synonym Gene Names
UBXN2C
UniProt Entry Name
NSF1C_HUMAN

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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