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ZFYVE16 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904744

Endofin may be involved in regulating membrane trafficking in the endosomal pathway. Overexpression of Endofin induces endosome aggregation. It is required to target TOM1 to endosomes [taken from the Universal Protein Resource (UniProt)… More details

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Product Overview

Online Datasheet in PDF
Product Name
Endofin (ZFYVE16), Polyclonal Antibody
Full Product Name
Rabbit anti-Endofin Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
endosome-associated FYVE-domain protein; zinc finger FYVE domain-containing protein 16; zinc finger, FYVE domain containing 16; Endofin; protein phosphatase 1, regulatory subunit 69; Endosome-associated FYVE domain protein; PPP1R69; Zinc finger FYVE domain-containing protein 16; zinc finger FYVE-type containing 16
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
608880
3D Structure
ModBase 3D Structure for Q7Z3T8
Immunogen
Between 450 and 500
Conjugation
Unconjugated

Product Categories/Family for anti-ZFYVE16 antibody

Applications Tested/Suitable for anti-ZFYVE16 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-ZFYVE16 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-ZFYVE16 antibody

Detection of human Endofin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Endofin antibody MBS3904744 (lot MBS3904744-1) used for IP at 6 ug per reaction. Endofin was also immunoprecipitated by rabbit anti-Endofin antibody MBS3904745. For blotting immunoprecipitated Endofin, MBS3904744 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-ZFYVE16 Polyclonal Antibody – Cat# MBS3904744

Western Blot (WB) of anti-ZFYVE16 antibody

Detection of human Endofin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Endofin antibody MBS3904744 (lot MBS3904744-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-ZFYVE16 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904744

Other Notes

Small volumes of anti-ZFYVE16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZFYVE16 purchase

MBS3904744 is an antibody targeting ZFYVE16. Also known as Rabbit anti-Endofin Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ZFYVE16. It may not necessarily be applicable to this product.

NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Protein Name
Zinc finger FYVE domain-containing protein 16
UniProt Synonym Protein Names
Endofin; Endosome-associated FYVE domain protein
UniProt Gene Name
ZFYVE16
UniProt Synonym Gene Names
KIAA0305
UniProt Entry Name
ZFY16_HUMAN

UniProt Comments for ZFYVE16

endofin: May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 5q14

Cellular Component: intracellular membrane-bound organelle; early endosome membrane; early endosome; cytoplasm

Molecular Function: protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein transporter activity; phosphatidylinositol binding

Biological Process: BMP signaling pathway; protein targeting to lysosome; vesicle organization and biogenesis; regulation of endocytosis; signal transduction; endosome transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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