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MIOS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904865

MIOS is a component of the GATOR2 complex. It inhibits GATOR1 complex, an inhibitor of the amino acid-sensing branch of the TORC1 pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NXC5]. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MIOS, Polyclonal Antibody
Full Product Name
Rabbit anti-MIOS Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
missing oocyte, meiosis regulator, homolog; Sea4; Yulink; WUGSC:H_DJ1159O04.1; Missing oocyte meiosis regulator homolog; GATOR complex protein MIOS; MIO; H_DJ1159O04.1; WD repeat-containing protein mio; WD repeat-containing protein mio; meiosis regulator for oocyte development
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
98,584 Da
OMIM
615359
3D Structure
ModBase 3D Structure for Q9NXC5
Predicted Reactivity
Orangutan
Immunogen
Between 825 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-MIOS antibody

Applications Tested/Suitable for anti-MIOS antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-MIOS antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-MIOS antibody

Detection of human MIOS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody MBS3904865 (lot MBS3904865-1) used for IP at 6 ug per reaction. MIOS was also immunoprecipitated by rabbit anti-MIOS antibody BL18122. For blotting immunoprecipitated MIOS, MBS3904865 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-MIOS Polyclonal Antibody – Cat# MBS3904865

Western Blot (WB) of anti-MIOS antibody

Detection of human and mouse MIOS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody MBS3904865 (lot MBS3904865-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-MIOS Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904865

Other Notes

Small volumes of anti-MIOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIOS purchase

MBS3904865 is an antibody targeting MIOS. Also known as Rabbit anti-MIOS Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MIOS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
WD repeat-containing protein mio
NCBI Official Synonym Full Names
missing oocyte, meiosis regulator, homolog (Drosophila)
NCBI Official Symbol
MIOS
UniProt Protein Name
WD repeat-containing protein mio
UniProt Gene Name
MIOS
UniProt Entry Name
MIO_HUMAN
NCBI Protein Information
WD repeat-containing protein mio; H_DJ1159O04.1; WUGSC:H_DJ1159O04.1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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