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RPL4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905194

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL4 is a ribosomal component of the… More details

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Product Overview

Online Datasheet in PDF
Product Name
RPL4/Ribosomal Protein L4, Polyclonal Antibody
Full Product Name
Rabbit anti-RPL4/Ribosomal Protein L4 Antibody, Affinity Purified
Product Gene Name
anti-RPL4 antibody
Product Synonym Names
60S ribosomal protein L1; 60S ribosomal protein L4; L4; large ribosomal subunit protein uL4; 60S ribosomal protein L4; ribosomal protein L4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
47,697 Da
OMIM
180479
3D Structure
ModBase 3D Structure for P36578
Predicted Reactivity
Orangutan
Immunogen
Between 377 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-RPL4 antibody

Primary Antibodies
Applications Tested/Suitable for anti-RPL4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-RPL4 antibody
WB: 1:10,000-1:25,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-RPL4 antibody

Detection of human RPL4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL4 antibody (MBS3905194 lot 1) used for IP at 6 ug per reaction. RPL4 was also immunoprecipitated by a second antibody against a different epitope of RPL4 (BL19864). For blotting immunoprecipitated RPL4, MBS3905194 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-RPL4 Polyclonal Antibody – Cat# MBS3905194

Western Blot (WB) of anti-RPL4 antibody

Detection of human RPL4 by western blot. Samples: Whole cell lysate (10 ug) from MCF-7, RKO, LNCaP, K-562, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL4 antibody (MBS3905194 lot 1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-RPL4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905194

Other Notes

Small volumes of anti-RPL4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPL4 purchase

MBS3905194 is an antibody targeting RPL4. Also known as Rabbit anti-RPL4/Ribosomal Protein L4 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPL4. It may not necessarily be applicable to this product.

NCBI GI #
16579885
NCBI GeneID
6124
NCBI Accession #
NP_000959.2
UniProt Primary Accession #
P36578
UniProt Secondary Accession #
P39029; Q4VBR0; Q969Z9; A8K502
UniProt Related Accession #
P36578
GenBank Nucleotide #
NM_000968.3
NCBI Official Full Name
60S ribosomal protein L4
NCBI Official Synonym Full Names
ribosomal protein L4
NCBI Official Symbol
RPL4
NCBI Official Synonym Symbols
L4
UniProt Protein Name
60S ribosomal protein L4
UniProt Synonym Protein Names
60S ribosomal protein L1
Protein Family
60S ribosomal protein
UniProt Gene Name
RPL4
UniProt Synonym Gene Names
RPL1
UniProt Entry Name
RL4_HUMAN
NCBI Protein Information
60S ribosomal protein L4; 60S ribosomal protein L1

NCBI Summary for RPL4

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
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UniProt Comments for RPL4

RPL4: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle
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Research Articles on RPL4

1. Mouse and human RPL4 enhance MoMLV gag-pol readthrough.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPL4 antibody

 Reference Product  PubMed Publications
 RPS3 antibody  >4 publications with RPL4 and RPS3
 RPL6 antibody  >3 publications with RPL4 and RPL6
 RPL3 antibody  >3 publications with RPL4 and RPL3
 RPL5 antibody  >3 publications with RPL4 and RPL5
 RPL9 antibody  >3 publications with RPL4 and RPL9
 RPS14 antibody  >2 publications with RPL4 and RPS14
 RPS5 antibody  >1 publications with RPL4 and RPS5
 RPL11 antibody  >1 publications with RPL4 and RPL11

Pathways associated with anti-RPL4 antibody

 Products by Pathway  Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Cytoplasmic Ribosomal Proteins Pathway antibodies  Cytoplasmic Ribosomal Proteins Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Eukaryotic Translation Termination Pathway antibodies  Eukaryotic Translation Termination Pathway Diagram
 Formation Of A Pool Of Free 40S Subunits Pathway antibodies  Formation Of A Pool Of Free 40S Subunits Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Influenza Infection Pathway antibodies  Influenza Infection Pathway Diagram

Diseases associated with anti-RPL4 antibody

 Disease Name  Pubmed Publications
 Gastrointestinal Diseases Antibodies  >1 publications with RPL4 and Gastrointestinal Diseases
 Ovarian Neoplasms Antibodies  >1 publications with RPL4 and Ovarian Neoplasms
 Congenital Abnormalities Antibodies  >1 publications with RPL4 and Congenital Abnormalities
 Liver Diseases Antibodies  >1 publications with RPL4 and Liver Diseases
 Anemia Antibodies  >1 publications with RPL4 and Anemia
 Heart Diseases Antibodies  >1 publications with RPL4 and Heart Diseases
 Hepatitis Antibodies  >1 publications with RPL4 and Hepatitis
 Hyperplasia Antibodies  >1 publications with RPL4 and Hyperplasia
 Liver Neoplasms Antibodies  >1 publications with RPL4 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with RPL4 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-RPL4 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >5 publications with RPL4 and Liver
 Brain Antibodies  >5 publications with RPL4 and Brain
 Blood Antibodies  >3 publications with RPL4 and Blood
 Kidney Antibodies  >2 publications with RPL4 and Kidney
 Bone Antibodies  >2 publications with RPL4 and Bone
 Heart Antibodies  >2 publications with RPL4 and Heart
 Bone Marrow Antibodies  >2 publications with RPL4 and Bone Marrow
 Ovary Antibodies  >2 publications with RPL4 and Ovary
 Muscle Antibodies  >2 publications with RPL4 and Muscle
 Stomach Antibodies  >2 publications with RPL4 and Stomach
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