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SHMT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905487

Serine hydroxymethyltransferase (SHMT2) is the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. SHMT2 is primarily responsible… More details

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Product Overview

Online Datasheet in PDF
Product Name
SHMT2, Polyclonal Antibody
Full Product Name
Rabbit anti-SHMT2 Antibody, Affinity Purified
Product Gene Name
anti-SHMT2 antibody
Product Synonym Names
serine hydroxymethylase; SHMT; threonine aldolase; serine methylase; serine hydroxymethyltransferase, mitochondrial; serine hydroxymethyltransferase 2 (mitochondrial); HEL-S-51e; glycine hydroxymethyltransferase; glycine auxotroph A, human complement for hamster; GLYA; epididymis secretory sperm binding protein Li 51e; GLY A+; serine aldolase; Serine hydroxymethyltransferase, mitochondrial; serine hydroxymethyltransferase 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
138450
3D Structure
ModBase 3D Structure for P34897
Immunogen
Between 454 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-SHMT2 antibody

Primary Antibodies
Applications Tested/Suitable for anti-SHMT2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-SHMT2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-SHMT2 antibody

Detection of human SHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHMT2 antibody MBS3905487 (lot MBS3905487-1) used for IP at 6 ug per reaction. SHMT2 was also immunoprecipitated by rabbit anti-SHMT2 antibody A300-350A. For blotting immunoprecipitated SHMT2, MBS3905487 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-SHMT2 Polyclonal Antibody – Cat# MBS3905487

Western Blot (WB) of anti-SHMT2 antibody

Detection of human and mouse SHMT2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHMT2 antibody MBS3905487 (lot MBS3905487-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-SHMT2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905487

Other Notes

Small volumes of anti-SHMT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SHMT2 purchase

MBS3905487 is an antibody targeting SHMT2. Also known as Rabbit anti-SHMT2 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SHMT2. It may not necessarily be applicable to this product.

NCBI GeneID
6472
NCBI Accession #
NP_005403.2
UniProt Primary Accession #
P34897
UniProt Protein Name
Serine hydroxymethyltransferase, mitochondrial
UniProt Synonym Protein Names
Glycine hydroxymethyltransferase; Serine methylase
UniProt Gene Name
SHMT2
UniProt Synonym Gene Names
SHMT
UniProt Entry Name
GLYM_HUMAN

UniProt Comments for SHMT2

SHMT2: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; Energy Metabolism - methane; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; EC 2.1.2.1; Other Amino Acids Metabolism - cyanoamino acid

Chromosomal Location of Human Ortholog: 12q12-q14

Cellular Component: microtubule cytoskeleton; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space

Molecular Function: L-allo-threonine aldolase activity; identical protein binding; amino acid binding; glycine hydroxymethyltransferase activity; chromatin binding; pyridoxal phosphate binding

Biological Process: L-serine biosynthetic process; positive regulation of cell proliferation; one-carbon compound metabolic process; glycine biosynthetic process from serine; protein homotetramerization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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