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OAT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905491

Ornithine aminotransferase (OAT) is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal… More details

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Product Overview

Online Datasheet in PDF
Product Name
OAT, Polyclonal Antibody
Full Product Name
Rabbit anti-OAT Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
ornithine aminotransferase, mitochondrial; testicular tissue protein Li 130; ornithine-oxo-acid aminotransferase; Ornithine--oxo-acid aminotransferase; ornithine delta-aminotransferase; OATASE; HOGA; gyrate atrophy; GACR; OKT; Ornithine aminotransferase, mitochondrial; ornithine aminotransferase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
48,535 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (126085872..126107545, complement). Location: 10q26
OMIM
258870
3D Structure
ModBase 3D Structure for P04181
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-OAT antibody

Applications Tested/Suitable for anti-OAT antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-OAT antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-OAT antibody

Detection of human OAT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-OAT antibody MBS3905491 (lot MBS3905491-1) used for IP at 6 ug per reaction. OAT was also immunoprecipitated by rabbit anti-OAT antibody BL21203. For blotting immunoprecipitated OAT, MBS3905491 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-OAT Polyclonal Antibody – Cat# MBS3905491

Western Blot (WB) of anti-OAT antibody

Detection of human and mouse OAT by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-OAT antibody MBS3905491 (lot MBS3905491-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-OAT Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905491

Other Notes

Small volumes of anti-OAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OAT purchase

MBS3905491 is an antibody targeting OAT. Also known as Rabbit anti-OAT Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for OAT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ornithine aminotransferase, mitochondrial isoform 1
NCBI Official Synonym Full Names
ornithine aminotransferase
NCBI Official Symbol
OAT
NCBI Official Synonym Symbols
OKT; GACR; HOGA; OATASE
UniProt Protein Name
Ornithine aminotransferase, mitochondrial
UniProt Synonym Protein Names
Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase
UniProt Gene Name
OAT
UniProt Entry Name
OAT_HUMAN
NCBI Protein Information
ornithine aminotransferase, mitochondrial; gyrate atrophy; ornithine delta-aminotransferase; ornithine-oxo-acid aminotransferase

NCBI Summary for OAT

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]
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UniProt Comments for OAT

OAT: Defects in OAT are the cause of hyperornithinemia with gyrate atrophy of choroid and retina (HOGA). HOGA is a slowly progressive blinding autosomal recessive disorder. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Amino Acid Metabolism - arginine and proline; EC 2.6.1.13; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: ornithine-oxo-acid transaminase activity; pyridoxal phosphate binding

Biological Process: visual perception; amino acid biosynthetic process

Disease: Gyrate Atrophy Of Choroid And Retina
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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