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HAT1/KAT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905496

Histone acetyltransferase 1 (HAT1) is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains.… More details

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Product Overview

Online Datasheet in PDF
Product Name
HAT1/KAT1, Polyclonal Antibody
Full Product Name
Rabbit anti-HAT1/KAT1 Antibody, Affinity Purified
Product Gene Name
anti-HAT1/KAT1 antibody
Product Synonym Gene Name
HAT1
Product Synonym Names
histone acetyltransferase type B catalytic subunit; KAT1; Histone acetyltransferase 1; Histone acetyltransferase type B catalytic subunit; histone acetyltransferase 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
603053
3D Structure
ModBase 3D Structure for O14929
Predicted Reactivity
Rat
Immunogen
Between 369 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-HAT1/KAT1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-HAT1/KAT1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-HAT1/KAT1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-HAT1/KAT1 antibody

Detection of human HAT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HAT1 antibody MBS3905496 (lot MBS3905496-1) used for IP at 6 ug per reaction. HAT1 was also immunoprecipitated by rabbit anti-HAT1 antibody BL21249. For blotting immunoprecipitated HAT1, MBS3905496 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-HAT1/KAT1 Polyclonal Antibody – Cat# MBS3905496

Western Blot (WB) of anti-HAT1/KAT1 antibody

Detection of human and mouse HAT1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HAT1 antibody MBS3905496 (lot MBS3905496-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-HAT1/KAT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905496

Other Notes

Small volumes of anti-HAT1/KAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAT1/KAT1 purchase

MBS3905496 is an antibody targeting HAT1/KAT1. Also known as Rabbit anti-HAT1/KAT1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HAT1/KAT1. It may not necessarily be applicable to this product.

NCBI GeneID
8520
NCBI Accession #
NP_003633.2
UniProt Primary Accession #
O14929
UniProt Protein Name
Histone acetyltransferase type B catalytic subunit
UniProt Synonym Protein Names
Histone acetyltransferase 1
UniProt Gene Name
HAT1
UniProt Synonym Gene Names
KAT1
UniProt Entry Name
HAT1_HUMAN

UniProt Comments for HAT1/KAT1

HAT1: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. Belongs to the HAT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 2q31.2-q33.1

Cellular Component: nucleoplasm; nuclear matrix; protein complex; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; nucleus

Molecular Function: H4 histone acetyltransferase activity; protein binding; histone acetyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly; internal protein amino acid acetylation; DNA replication-dependent nucleosome assembly; DNA packaging; response to nutrient; chromatin silencing at telomere
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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