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NDUFA5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905596

NDUFA5 is a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. NDUFA5 localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone [taken from… More details

$250 / 0.01 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NDUFA5, Polyclonal Antibody
Full Product Name
Rabbit anti-NDUFA5 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
Complex I-13kD-B; UQOR13; ubiquinone reductase; type I dehydrogenase; NUFM; NADH-ubiquinone oxidoreductase 13 kDa-B subunit; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa; complex I subunit B13; complex I 13kDa subunit B; CI-13KD-B; CI-13kB; B13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; NADH:ubiquinone oxidoreductase subunit A5
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
601677
3D Structure
ModBase 3D Structure for Q16718
Predicted Reactivity
Orangutan, Gorilla, Chimpanzee
Immunogen
Between 66 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-NDUFA5 antibody

Applications Tested/Suitable for anti-NDUFA5 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-NDUFA5 antibody
WB: 1:1000-1:5000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-NDUFA5 antibody

Detection of human NDUFA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFA5 antibody MBS3905596 (lot MBS3905596-1) used for IP at 6 ug per reaction. NDUFA5 was also immunoprecipitated by rabbit anti-NDUFA5 antibody BL21734. For blotting immunoprecipitated NDUFA5, MBS3905596 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Rabbit Anti-NDUFA5 Polyclonal Antibody – Cat# MBS3905596

Western Blot (WB) of anti-NDUFA5 antibody

Detection of human and mouse NDUFA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1, cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NDUFA5 antibody MBS3905596 (lot MBS3905596-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-NDUFA5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905596

Other Notes

Small volumes of anti-NDUFA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDUFA5 purchase

MBS3905596 is an antibody targeting NDUFA5. Also known as Rabbit anti-NDUFA5 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NDUFA5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit A5
NCBI Official Symbol
NDUFA5
NCBI Official Synonym Symbols
B13; NUFM; UQOR13; CI-13kB; CI-13KD-B
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
UniProt Synonym Protein Names
Complex I subunit B13; Complex I-13kD-B; CI-13kD-B; NADH-ubiquinone oxidoreductase 13 kDa-B subunit
Protein Family
UniProt Gene Name
NDUFA5
UniProt Synonym Gene Names
CI-13kD-B
UniProt Entry Name
NDUA5_HUMAN
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

NCBI Summary for NDUFA5

This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. [provided by RefSeq, Apr 2014]
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UniProt Comments for NDUFA5

NDUFA5: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA5 subunit family.

Protein type: EC 1.6.5.3; Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 7q31.33

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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