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CHCHD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905629

CCoiled-coil-helix-coiled-coil-helix domain-containing protein 3 (CHCHD3) is an inner mitochondrial membrane scaffold protein. Absence of CHCHD3 affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth,… More details

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Product Overview

Online Datasheet in PDF
Product Name
CHCHD3, Polyclonal Antibody
Full Product Name
Rabbit anti-CHCHD3 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
MICOS complex subunit MIC19; protein phosphatase 1, regulatory subunit 22; PPP1R22; MINOS3; Mic19; coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial; Coiled-coil-helix-coiled-coil-helix domain-containing protein 3; mitochondrial inner membrane organizing system 3; MICOS complex subunit MIC19; coiled-coil-helix-coiled-coil-helix domain containing 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
26,152 Da
OMIM
613748
3D Structure
ModBase 3D Structure for Q9NX63
Immunogen
Between 177 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-CHCHD3 antibody

Applications Tested/Suitable for anti-CHCHD3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-CHCHD3 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-CHCHD3 antibody

Detection of human CHCHD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHCHD3 antibody MBS3905629 (lot MBS3905629-1) used for IP at 6 ug per reaction. CHCHD3 was also immunoprecipitated by rabbit anti-CHCHD3 antibody BL21538. For blotting immunoprecipitated CHCHD3, MBS3905629 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-CHCHD3 Polyclonal Antibody – Cat# MBS3905629

Western Blot (WB) of anti-CHCHD3 antibody

Detection of human and mouse CHCHD3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHCHD3 antibody MBS3905629 (lot MBS3905629-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-CHCHD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905629

Other Notes

Small volumes of anti-CHCHD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHCHD3 purchase

MBS3905629 is an antibody targeting CHCHD3. Also known as Rabbit anti-CHCHD3 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHCHD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
MICOS complex subunit MIC19
NCBI Official Synonym Full Names
coiled-coil-helix-coiled-coil-helix domain containing 3
NCBI Official Symbol
CHCHD3
NCBI Official Synonym Symbols
MINOS3; PPP1R22
UniProt Protein Name
MICOS complex subunit MIC19
UniProt Synonym Protein Names
Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
UniProt Gene Name
CHCHD3
UniProt Synonym Gene Names
MIC19; MINOS3
UniProt Entry Name
MIC19_HUMAN
NCBI Protein Information
MICOS complex subunit MIC19; coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial; mitochondrial inner membrane organizing system 3; protein phosphatase 1, regulatory subunit 22

UniProt Comments for CHCHD3

CHCHD3: May be a scaffolding protein that stabilizes protein complexes involved in maintaining mitochondrial crista architecture and protein import.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane; nucleus

Molecular Function: protein binding; protein complex scaffold; phosphatase binding

Biological Process: inner mitochondrial membrane organization and biogenesis; mitochondrial fusion; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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