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IARS2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905758

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IARS2, Polyclonal Antibody
Full Product Name
Rabbit anti-IARS2 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
ILERS; mitochondrial isoleucine tRNA synthetase; isoleucyl-tRNA synthetase, mitochondrial; Isoleucyl-tRNA synthetase; isoleucine-tRNA synthetase 2, mitochondrial; isoleucine tRNA ligase 2, mitochondrial; CAGSSS; isoleucine--tRNA ligase, mitochondrial; Isoleucine--tRNA ligase, mitochondrial; isoleucyl-tRNA synthetase 2, mitochondrial
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
113,792 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (220267455..220321383). Location: 1q41
OMIM
612801
3D Structure
ModBase 3D Structure for Q9NSE4
Immunogen
Between 962 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-IARS2 antibody

Applications Tested/Suitable for anti-IARS2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-IARS2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-IARS2 antibody

Detection of human IARS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IARS2 antibody (MBS3905758 lot 1) used for IP at 2 ug per reaction. IARS2 was also immunoprecipitated by rabbit anti-IARS2 antibody BL22461. For blotting immunoprecipitated IARS2, MBS3905758 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-IARS2 Polyclonal Antibody – Cat# MBS3905758

Western Blot (WB) of anti-IARS2 antibody

Detection of human IARS2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IARS2 antibody (MBS3905758 lot 1) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-IARS2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905758

Other Notes

Small volumes of anti-IARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IARS2 purchase

MBS3905758 is an antibody targeting IARS2. Also known as Rabbit anti-IARS2 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IARS2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
isoleucine--tRNA ligase, mitochondrial
NCBI Official Synonym Full Names
isoleucyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
IARS2
NCBI Official Synonym Symbols
ILERS
UniProt Protein Name
Isoleucine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names
Isoleucyl-tRNA synthetase
UniProt Gene Name
IARS2
UniProt Synonym Gene Names
IleRS
UniProt Entry Name
SYIM_HUMAN
NCBI Protein Information
isoleucine--tRNA ligase, mitochondrial; isoleucine tRNA ligase 2, mitochondrial; isoleucyl-tRNA synthetase, mitochondrial; mitochondrial isoleucine tRNA synthetase; isoleucine-tRNA synthetase 2, mitochondrial

NCBI Summary for IARS2

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014]
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UniProt Comments for IARS2

IARS2: Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: Ligase; EC 6.1.1.5; Mitochondrial; Translation; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; cytosol

Molecular Function: ATP binding; isoleucine-tRNA ligase activity

Biological Process: isoleucyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; mitochondrial translation; regulation of translational fidelity; gene expression

Disease: Cataracts, Growth Hormone Deficiency, Sensory Neuropathy, Sensorineural Hearing Loss, And Skeletal Dysplasia
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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