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Myosin Light Chain 1 (MYL1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3906106

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two non-regulatory alkali light chains, and two regulatory light chains. Myosin Light Chain 1 is a myosin alkali light chain expressed in fast skeletal muscle. [taken… More details

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Product Overview

Online Datasheet in PDF
Product Name
Myosin Light Chain 1 (MYL1), Monoclonal Antibody
Full Product Name
Mouse anti-Myosin Light Chain 1 Monoclonal Antibody [39-15]
Product Gene Name
Product Synonym Names
myosin light chain A1/A2; A1 catalytic; skeletal, fast; myosin, light polypeptide 1, alkali; myosin, light chain 1, alkali; myosin light chain alkali 1/2; myosin light chain 1/3, skeletal muscle isoform; MLC3F; MLC1F/MLC3F; MLC1F; A2 catalytic; MLC1/MLC3; Myosin light chain 1/3, skeletal muscle isoform; myosin light chain 1
Clonality
Monoclonal
Clone Number
39-15
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Purified
Concentration
100ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
21,145 Da
OMIM
160780
3D Structure
ModBase 3D Structure for P05976
Immunogen
Human Myosin Light Chain 1
Conjugation
Unconjugated

Product Categories/Family for anti-MYL1 antibody

Applications Tested/Suitable for anti-MYL1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MYL1 antibody
WB: 1:1,000
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-MYL1 antibody

Detection of human Myosin Light Chain 1 by immunohistochemistry. Sample: FFPE section of human skeletal muscle. Antibody: Mouse anti-Myosin Light Chain 1 monoclonal antibody [39-15)] (MBS3906106 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG (MBS3907120).
Mouse Anti-MYL1 Monoclonal Antibody – Cat# MBS3906106

Immunohistochemistry (IHC) of anti-MYL1 antibody

Detection of human Myosin Light Chain 1 by immunohistochemistry. Sample: FFPE section of human cardiac muscle. Antibody: Mouse anti-Myosin Light Chain 1 monoclonal antibody [39-15)] (MBS3906106 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG (MBS3907120).
Mouse Anti-MYL1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3906106

Western Blot (WB) of anti-MYL1 antibody

Detection of human Myosin Light Chain 1 by western blot. Samples: Tissue lysate (50 ug) from human skeletal muscle and heart. Antibody: Mouse anti-Myosin Light Chain 1 monoclonal antibody [39-15] (MBS3906106 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (MBS3907120). Detection: Chemiluminescence with an exposure time of 3 seconds.
Mouse Anti-MYL1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS3906106

Western Blot (WB) of anti-MYL1 antibody

Detection of mouse Myosin Light Chain 1 by western blot. Samples: Tissue lysate (50 ug) from mouse skeletal muscle and heart. Antibody: Mouse anti-Myosin Light Chain 1 monoclonal antibody [39-15] (MBS3906106 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (MBS3907120). Detection: Chemiluminescence with an exposure time of 3 seconds.
Mouse Anti-MYL1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS3906106

Other Notes

Small volumes of anti-MYL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYL1 purchase

MBS3906106 is an antibody targeting MYL1. Also known as Mouse anti-Myosin Light Chain 1 Monoclonal Antibody [39-15]. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MYL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
myosin light chain 1/3, skeletal muscle isoform isoform 1f
NCBI Official Synonym Full Names
myosin, light chain 1, alkali; skeletal, fast
NCBI Official Symbol
MYL1
NCBI Official Synonym Symbols
MLC1F; MLC3F
UniProt Protein Name
Myosin light chain 1/3, skeletal muscle isoform
UniProt Synonym Protein Names
Myosin light chain alkali 1/2
Protein Family
UniProt Gene Name
MYL1
UniProt Synonym Gene Names
MLC1/MLC3; MLC1F/MLC3F
UniProt Entry Name
MYL1_HUMAN
NCBI Protein Information
myosin light chain 1/3, skeletal muscle isoform; MLC1/MLC3; MLC1F/MLC3F; A1 catalytic; A2 catalytic; myosin light chain A1/A2; myosin light chain alkali 1/2; myosin, light polypeptide 1, alkali; skeletal, fast

NCBI Summary for MYL1

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for MYL1

MYL1: Regulatory light chain of myosin. Does not bind calcium. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: sarcomere; myofibril; muscle myosin complex; cytosol

Molecular Function: structural constituent of muscle; calcium ion binding

Biological Process: muscle contraction; muscle filament sliding; cardiac muscle contraction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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