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eIF2 alpha Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9503010

MBS9503010 is an antibody targeting eIF2 alpha. Also known as eIF2 alpha (Phospho-Ser51) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
eIF2 alpha, Polyclonal Antibody
Full Product Name
eIF2 alpha (Phospho-Ser51) Antibody
Product Gene Name
anti-eIF2 alpha antibody
Product Synonym Gene Name
EIF2S1
Product Synonym Names
eIF-2-alpha; EIF-2A; EIF-2alpha; EIF2S1; Eukaryotic translation initiation factor 2 alpha subunit; Eukaryotic translation initiation factor 2 subunit 1; IF2A
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
1 mg/ml (lot specific)
Specificity
eIF2 alpha (Phospho-Ser51) Antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Ser51.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Molecular Weight
36 kDa
OMIM
J02645 mRNA
3D Structure
ModBase 3D Structure for P05198
Target Modification
Phospho
Modification Sites
H:S52; M:S52; R:S52
Immunogen
The antiserum was produced against synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51.
Immunogen Range
21-70
Applications Tested/Suitable for anti-eIF2 alpha antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-eIF2 alpha antibody
WB: 1:500~1:1000
ELISA: 1:10000

Western Blot (WB) of anti-eIF2 alpha antibody

Western blot analysis of lysates from K562 cells treated with IFN-alpha 1000U/ml 18h, using eIF2 alpha (Phospho-Ser51) Antibody. The lane on the right is blocked with the phospho peptide.
Rabbit Anti-eIF2 alpha Polyclonal Antibody – Cat# MBS9503010

Other Notes

Small volumes of anti-eIF2 alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for eIF2 alpha. It may not necessarily be applicable to this product.

NCBI GI #
4758256
NCBI GeneID
1965
NCBI Accession #
NP_004085.1
UniProt Primary Accession #
P05198
UniProt Related Accession #
P05198
GenBank Nucleotide #
NM_004094.4
NCBI Official Full Name
eukaryotic translation initiation factor 2 subunit 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 subunit alpha
NCBI Official Symbol
EIF2S1
NCBI Official Synonym Symbols
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
UniProt Protein Name
Eukaryotic translation initiation factor 2 subunit 1
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
Protein Family
Eukaryotic translation initiation factor
UniProt Gene Name
EIF2S1
UniProt Synonym Gene Names
EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha
NCBI Protein Information
eukaryotic translation initiation factor 2 subunit 1

NCBI Summary for eIF2 alpha

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
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UniProt Comments for eIF2 alpha

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
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Research Articles on eIF2 alpha

1. a novel, positive role for PKR activation and eIF2alpha phosphorylation in human globin mRNA splicing, is reported.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-eIF2 alpha antibody

 Reference Product  PubMed Publications
 EIF2AK3 antibody  >1 publications with eIF2 alpha and EIF2AK3
 EIF2AK2 antibody  >1 publications with eIF2 alpha and EIF2AK2

Pathways associated with anti-eIF2 alpha antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies  Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram

Diseases associated with anti-eIF2 alpha antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >2 publications with eIF2 alpha and Death
 Hyperglycemia Antibodies  >1 publications with eIF2 alpha and Hyperglycemia
 Glucose Intolerance Antibodies  >1 publications with eIF2 alpha and Glucose Intolerance

Organs/Tissues associated with anti-eIF2 alpha antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >1 publications with eIF2 alpha and Pancreas
 Blood Antibodies  >1 publications with eIF2 alpha and Blood
 Brain Antibodies  >1 publications with eIF2 alpha and Brain
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