Mouse Anti-p53 Monoclonal Antibody – Cat# MBS395100

Western blot analysis using p53 cln HR231 antibody at 1 ug/ml on native H1299 cells (1) and H1299 cells transfected with human p53. Also shown is a graphic representation of the epitope location.

p53 Antibody – Mouse Monoclonal

Name: p53 Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS395100
Price: 500 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS395100

p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in… More details

$500 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

p53 (a.a. 371-380), Monoclonal Antibody

Full Product Name

p53 (a.a. 371-380)

Product Gene Name

anti-p53 antibody

Clonality

Monoclonal

Isotype

IgG2b

Clone Number

HR231

Host

Mouse

Species Reactivity

Human, Monkey, Mouse, Rat

Form/Format

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purity/Purification

Protein A/G Chromatography

Preparation and Storage

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Molecular Weight

40,635 Da

Chromosome Location

Chromosome: 10; NC_000076.6 (61715263..61739260). Location: 10 B4; 10

3D Structure

ModBase 3D Structure for Q8BUE4

Immunogen

Hybridoma produced by the fusion of splenocytes from immunized with full length human p53 protein and mouse myeloma cells.

Positive Control

Breast or colon carcinoma cells

Product Categories/Family for anti-p53 antibody

Monoclonal Antibody; p53; and Related/Induced Proteins

Applications Tested/Suitable for anti-p53 antibody

ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Other Notes

Small volumes of anti-p53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p53 purchase

MBS395100 is an antibody targeting p53. Also known as p53 (a.a. 371-380). Reactive in Human, Monkey, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for p53. It may not necessarily be applicable to this product.

NCBI GI #

85861162

NCBI GeneID

71361

NCBI Accession #

NP_001034283.1

UniProt Primary Accession #

Q8BUE4

UniProt Secondary Accession #

Q8CHZ2

UniProt Related Accession #

Q8BUE4

GenBank Nucleotide #

NM_001039194.3

NCBI Official Full Name

apoptosis-inducing factor 2 isoform 2

NCBI Official Synonym Full Names

apoptosis-inducing factor, mitochondrion-associated 2

NCBI Official Symbol

Aifm2

NCBI Official Synonym Symbols

Amid; PRG3; 5430437E11Rik; D730001I10Rik

UniProt Protein Name

Apoptosis-inducing factor 2

UniProt Synonym Protein Names

Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death

UniProt Gene Name

Aifm2

UniProt Synonym Gene Names

Amid

UniProt Entry Name

AIFM2_MOUSE

NCBI Protein Information

apoptosis-inducing factor 2; apoptosis-inducing factor-like mitchondrion-associated inducer of death; apoptosis-inducing factor-like mitochondrion-associated inducer of death; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; apoptosis-inducing factor homologous mitochondrion-associated inducer of death

UniProt Comments for p53

Function: Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death

By similarity. May contribute to genotoxin-induced growth arrest.

Cofactor: FAD. Binds 6-hydroxy-FAD non-covalently

By similarity.

Subcellular location: Cytoplasm. Mitochondrion outer membrane

By similarity.

Tissue specificity: Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in liver, testis, and kidney, and expressed at lower levels in pancreas, spleen, brain and lung. Ref.4

Induction: Expression is up-regulated in mouse embryonic fibroblasts by genotoxic reagents 5-fluorouracil and etoposide. Ref.4

Sequence similarities: Belongs to the FAD-dependent oxidoreductase family.

Sequence caution: Isoform 2: The sequence AAH38129.1 differs from that shown. Reason: erroneous termination (Translated as Trp in position: 339:Stop->W)

Research Articles on p53

1. Results demonstrate nuclear AIF translocation only in PARG-null TS cells, which demonstrates the presence of AIF-mediated cell death.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-p53 antibody

Reference Product	PubMed Publications
Trp53 antibody	>3 publications with p53 and Trp53

Organs/Tissues associated with anti-p53 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1 publications with p53 and Lung
Kidney Antibodies	>1 publications with p53 and Kidney
Brain Antibodies	>1 publications with p53 and Brain
Embryonic Tissue Antibodies	>1 publications with p53 and Embryonic Tissue