DNA Adenine Methylase (dam) Antibody – Mouse Monoclonal

DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent. Antibody can be used for Western blotting (1-5 µg/ml). Other applications not yet evaluated. Optimal concentration should be evaluated by serial dilutions. The antibody reacts with the recombinant protein as produced by E.coli. We have no data to support to its reactivity with wild type DAM protein.

Small volumes of anti-dam antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS395227 is an antibody targeting dam. Also known as DNA Adenine Methylase. Reactive in Gram Negative Bacteria. Host: Mouse.