SSRP1 NT Antibody – Rabbit Polyclonal

Function: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA. Also acts as a transcriptional coactivator for p63/TP63.

Subunit structure: Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Binds to histone H3-H4 tetramers, but not to intact nucleosomes. Interacts with isoform gamma of p63/TP63. Interacts with FYTTD1/UIF, SRF and NEK9

By similarity.

Subcellular location: Nucleus. Nucleus › nucleolus

By similarity. Chromosome. Note: Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci

By similarity.

Post-translational modification: Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity

By similarity.Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein

By similarity.Sumoylated

By similarity.

Disruption phenotype: Embryos die soon after implantation while preimplantation blastocysts are defective for cell outgrowth. Ref.4

Sequence similarities: Belongs to the SSRP1 family.Contains 1 HMG box DNA-binding domain.

Sequence caution: The sequence AAH42502.1 differs from that shown. Reason: Frameshift at position 697.