RIN Antibody – Rabbit Polyclonal

Function: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis. Ref.3 Ref.5 Ref.8

Subunit structure: Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351. Ref.5 Ref.8

Subcellular location: Cytoplasm. Membrane. Cytoplasm › cytoskeleton. Note: Some amount is membrane-associated. Ref.7

Tissue specificity: Expressed in all tissues examined with high levels in brain, placenta and pancreas. Ref.3

Post-translational modification: Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylation at Ser-351 by PRKD1 induces interaction with 14-3-3 proteins. Ref.3 Ref.5 Ref.7 Ref.8

Sequence similarities: Belongs to the RIN (Ras interaction/interference) family.Contains 1 Ras-associating domain.Contains 1 SH2 domain.Contains 1 VPS9 domain.