Sheep Anti-VAV-2 DPH Polyclonal Antibody – Cat# MBS395620

Legend:HEK293 cells were transfected with either emply expression vector (pSR) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 ul/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 ul/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 ul/300ug lysate). DPH or SHD anti Vav2 antisera (2.5ul/300ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.

VAV-2 DPH Antibody – Sheep Polyclonal

Name: VAV-2 DPH Antibody; Polyclonal; Sheep; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS395620
Price: 500 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS395620

The immunogen used for the anti-human Vav-2 DPH region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50% homology at the… More details

$500 / 0.25 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Vav-2 DPH, Polyclonal Antibody

Full Product Name

Vav-2 DPH

Product Gene Name

anti-Vav-2 DPH antibody

Product Synonym Names

VAV-2; Guanine nucleotide exchange factor VAV-2; Dbl homology (DH) domain

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Human

Form/Format

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purity/Purification

Ammonium Sulfate Precipitation

Preparation and Storage

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Molecular Weight

101

Chromosome Location

Chromosome: 9; NC_000009.11 (136627016..136857446, complement). Location: 9q34.1

OMIM

600428

3D Structure

ModBase 3D Structure for P52735

Immunogen

A his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a molecular weight of 100kD

Product Categories/Family for anti-Vav-2 DPH antibody

Polyclonal Antibody; Signaling Intermediates

Applications Tested/Suitable for anti-Vav-2 DPH antibody

Western Blot (WB), Immunoprecipitation (IP)

Other Notes

Small volumes of anti-Vav-2 DPH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Vav-2 DPH purchase

MBS395620 is an antibody targeting Vav-2 DPH. Reactive in Human. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for Vav-2 DPH. It may not necessarily be applicable to this product.

NCBI GI #

197304715

NCBI GeneID

7410

NCBI Accession #

NP_001127870.1

UniProt Primary Accession #

P52735

UniProt Secondary Accession #

Q5SYV3; Q5SYV4; Q5SYV5; Q6N012; Q6PIJ9; Q6Q317; A2RUM4; A8MQ12; B6ZDF5

UniProt Related Accession #

P52735

GenBank Nucleotide #

NM_001134398.1

NCBI Official Full Name

guanine nucleotide exchange factor VAV2 isoform 1

NCBI Official Synonym Full Names

vav 2 guanine nucleotide exchange factor

NCBI Official Symbol

VAV2

NCBI Official Synonym Symbols

VAV-2

UniProt Protein Name

Guanine nucleotide exchange factor VAV2

UniProt Gene Name

VAV2

UniProt Synonym Gene Names

VAV-2

UniProt Entry Name

VAV2_HUMAN

NCBI Protein Information

guanine nucleotide exchange factor VAV2; vav 2 oncogene

NCBI Summary for Vav-2 DPH

VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

UniProt Comments for Vav-2 DPH

Function: Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly

By similarity.

Subunit structure: Interacts (via SH2 domains) with the phosphorylated form of EPHA2

By similarity. Interacts with NEK3 and PRLR and this interaction is prolactin-dependent. Ref.9

Tissue specificity: Widely expressed.

Post-translational modification: Phosphorylated on tyrosine residues in response to FGR activation. Ref.7 Ref.8

Sequence similarities: Contains 1 CH (calponin-homology) domain.Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 SH2 domain.Contains 2 SH3 domains.

Research Articles on Vav-2 DPH

1. Studies indicate relevance of P-Rex1 and P-Rex2a, in breast tumorigenesis, and suggest that the exchange factors Vav2 and Vav3 play synergistic roles in breast cancer by sustaining tumor growth, neoangiogenesis, and metastasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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Diseases associated with anti-Vav-2 DPH antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>36 publications with Vav-2 DPH and Neoplasms
Cell Transformation, Neoplastic Antibodies	>7 publications with Vav-2 DPH and Cell Transformation, Neoplastic
Neoplasms, Experimental Antibodies	>5 publications with Vav-2 DPH and Neoplasms, Experimental
Inflammation Antibodies	>4 publications with Vav-2 DPH and Inflammation
Adenocarcinoma Antibodies	>4 publications with Vav-2 DPH and Adenocarcinoma
Lung Neoplasms Antibodies	>4 publications with Vav-2 DPH and Lung Neoplasms
Liver Neoplasms Antibodies	>3 publications with Vav-2 DPH and Liver Neoplasms
Kidney Diseases Antibodies	>2 publications with Vav-2 DPH and Kidney Diseases
Carcinoma, Squamous Cell Antibodies	>2 publications with Vav-2 DPH and Carcinoma, Squamous Cell
Weight Loss Antibodies	>2 publications with Vav-2 DPH and Weight Loss