p73 Antibody – Rabbit Polyclonal

This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq, Jul 2008]

Function: Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Ref.6 Ref.7 UniProtKB O15350

Cofactor: Binds 1 zinc ion per subunit

By similarity. UniProtKB O15350

Subunit structure: Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2, HIPK2, RANBP9 and WWOX

By similarity. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain)

By similarity. Interacts with YAP1 (phosphorylated form)

By similarity. Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation

By similarity. UniProtKB O15350

Subcellular location: Nucleus. Cytoplasm

By similarity. Note: Accumulates in the nucleus in response to DNA damage. Ref.8

Tissue specificity: Found in striatal neurons of mutant huntingtin (htt) transgenic mice (at protein level). Isoform 1 is expressed in the nasal epithelium, the vomeronasal organ, the hippocampus and the hypothalamus. Ref.1 Ref.9

Domain: Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL1 tyrosine kinase SH3 domain

By similarity. UniProtKB O15350The WW-binding motif mediates interaction with WWOX

By similarity. UniProtKB O15350

Post-translational modification: Sumoylated on Lys-622, which potentiates proteasomal degradation but does not affect transcriptional activity

By similarity.Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function

By similarity. Higher levels of phosphorylation seen in striatal neurons of. mutant huntingtin (htt) transgenic mice.Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome

By similarity.

Disruption phenotype: Mice lacking Tp73 display a runting phenotype and high rates of mortality due to massive gastrointestinal hemorrhages or intracranial bleeding. The gastrointestinal tract suffers loss of enterocytes and excessive mucosecretions in the duodenum, ileum and cecum. Survivors exhibit hippocampal dysgenesis, hydrocephalus, chronic infections and inflammation, as well as abnormalities in pheromone sensory pathways. Ref.1

Miscellaneous: Activated and stabilized by interaction with RANBP9

By similarity. UniProtKB O15350

Sequence similarities: Belongs to the p53 family. UniProtKB O15350Contains 1 SAM (sterile alpha motif) domain.

Sequence caution: The sequence CAM18775.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.