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N-Smase Activation Associated Factor (FAN) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS395673

Novel adaptor protein which mediates the activation of sphingomyelinase by receptors such as tumor necrosis factor receptor type 1 and CD40 and subsequent apoptosis in cardiomyocytes. Inhibition of this factor may provide a novel intervention point for… More details

$500 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FAN, N-SMase Activation Associated Factor, Polyclonal Antibody
Full Product Name
FAN, N-SMase Activation Associated Factor
Product Gene Name
Product Synonym Names
FAN (Factor associated with neutral sphingomyelinase activation) SMAF; Neutral sphingomyelinase (N-SMase) activation associated factor
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purity/Purification
Ammonium Sulfate Precipitation
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Molecular Weight
104 kDa
Chromosome Location
Chromosome: 8; NC_000008.10 (59496063..59572404, complement). Location: 8q12-q13
OMIM
603043
3D Structure
ModBase 3D Structure for Q8IW26
Immunogen
Synthetic peptide derived from human NSMAF protein.
Positive Control
Human testis, brain

Product Categories/Family for anti-FAN antibody

Applications Tested/Suitable for anti-FAN antibody
Western Blot (WB)

Western Blot/Blotting of anti-FAN antibody

Western blot using anti nSMAF antibody on human brain lysate (A) and RMS-13 rhabdosarcoma cell lysate (B). Lysate used at 15 ug/lane. Antibody used at 1:400 dilution. Secondary antibody, mouse anti-rabbit HRP , used at 1:50k dilution. Visualized using Pierce West Femto substrate system. Exposure for 5 minutes
Rabbit Anti-FAN Polyclonal Antibody – Cat# MBS395673

Other Notes

Small volumes of anti-FAN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAN purchase

MBS395673 is an antibody targeting FAN. Also known as FAN, N-SMase Activation Associated Factor. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FAN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein FAN isoform 2
NCBI Official Synonym Full Names
neutral sphingomyelinase (N-SMase) activation associated factor
NCBI Official Symbol
NSMAF
NCBI Official Synonym Symbols
FAN
UniProt Protein Name
Protein FAN
UniProt Synonym Protein Names
Factor associated with neutral sphingomyelinase activation
UniProt Gene Name
NSMAF
UniProt Synonym Gene Names
FAN; Factor associated with N-SMase activation
UniProt Entry Name
FAN_HUMAN
NCBI Protein Information
protein FAN; factor associated with N-SMase activation; factor associated with neutral sphingomyelinase activation

NCBI Summary for FAN

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]
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UniProt Comments for FAN

Function: Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.

Tissue specificity: Ubiquitous.

Sequence similarities: Contains 1 BEACH domain.Contains 1 GRAM domain.Contains 6 WD repeats.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FAN antibody

 Reference Product  PubMed Publications
 CD40 antibody  >1 publications with FAN and CD40
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