• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Sphingosine Kinase 2, Long Form, Human (SPHK2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS395712

Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine… More details

$670 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Sphingosine Kinase 2, Long Form, Human (SPHK2), Polyclonal Antibody
Full Product Name
Sphingosine Kinase 2, Long Form, Human
Product Gene Name
Product Synonym Names
Sphk2-L; N-terminal-extended Sphingosine Kinase 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Formulation: Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide.
Form: Affinity Purified
Purity/Purification
Antigen Immunoaffiinity Purification
Preparation and Storage
Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles.
Shipping: Ships on blue ice.
Products are stable for 1 year from purchase when stored properly.
Molecular Weight
69,217 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (49122548..49133663). Location: 19q13.2
OMIM
607092
3D Structure
ModBase 3D Structure for Q9NRA0
Immunogen
Synthetic peptide derived from the N-terminal extension of the Sphingosine Kinase 2-L protein.
Positive Control
Jurkat lysate used as positive control.
Applications Tested/Suitable for anti-SPHK2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-SPHK2 antibody
Antibody can be used for Western blotting (1-5ug/ml)
Optimal concentration should be evaluated by serial dilutions.

Western Blot/Blotting of anti-SPHK2 antibody

Western blot of endogneous sphingosine kinase 2 LF detection in Jurkat cell lysate (4 ug/lane) using X1880P (1 ug/ml) developed using anti-rabbit HRp (1:75K) and Pierce Super Signal west Femto.
Rabbit Anti-SPHK2 Polyclonal Antibody – Cat# MBS395712

Other Notes

Small volumes of anti-SPHK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPHK2 purchase

MBS395712 is an antibody targeting SPHK2. Also known as Sphingosine Kinase 2, Long Form, Human. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPHK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sphingosine kinase 2 isoform a
NCBI Official Synonym Full Names
sphingosine kinase 2
NCBI Official Symbol
SPHK2
NCBI Official Synonym Symbols
SK 2; SK-2; SPK 2; SPK-2
UniProt Protein Name
Sphingosine kinase 2
Protein Family
UniProt Gene Name
SPHK2
UniProt Synonym Gene Names
SK 2; SPK 2
UniProt Entry Name
SPHK2_HUMAN
NCBI Protein Information
sphingosine kinase 2; sphingosine kinase type 2

NCBI Summary for SPHK2

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Show more

UniProt Comments for SPHK2

Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. Ref.8

Catalytic activity: ATP + sphinganine = ADP + sphinganine 1-phosphate. Ref.1ATP + sphingosine = ADP + sphingosine 1-phosphate. Ref.1

Cofactor: Magnesium

By similarity.

Enzyme regulation: Inhibited by sulfatide. Ref.8

Subcellular location: Isoform 1: Cytoplasm. Membrane Ref.6 Ref.8. Isoform 2: Lysosome membrane Ref.6 Ref.8.

Sequence similarities: Contains 1 DAGKc domain.

Biophysicochemical propertiesKinetic parameters:KM=3.4 µM for sphingosine Ref.1pH dependence:Optimum pH is 7.5.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPHK2 antibody

 Reference Product  PubMed Publications
 SGPP1 antibody  >2 publications with SPHK2 and SGPP1
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000