Rabbit Anti-TIRAP Polyclonal Antibody – Cat# MBS395869

Western blot analysis of TIRAP in human heart cell lysates with TIRAP antibody at (A) 0.5, (B) 1, and (C) 2 g/ml.

Toll Interleukin 1 Receptor Adaptor Protein (TIRAP) Antibody – Rabbit Polyclonal

Name: TIRAP Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS395869
Price: 720 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS395869

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as TIRAP… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Toll Interleukin 1 receptor adaptor protein (TIRAP), Polyclonal Antibody

Full Product Name

Toll Interleukin 1 receptor adaptor protein (TIRAP) (CT)

Product Gene Name

anti-TIRAP antibody

Product Synonym Names

MyD88 adapter-like protein; Adaptor protein Wyatt

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Provided as solution in phosphate buffered saline with 0.02% sodium azide

Purity/Purification

Antigen Immunoaffiinity Purification

Preparation and Storage

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Molecular Weight

26 kD

Chromosome Location

Chromosome: 9; NC_000075.6 (35184391..35200291, complement). Location: 9; 9 A5.3

3D Structure

ModBase 3D Structure for Q99JY1

Immunogen

Rabbit polyclonal TIRAP antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of mouse TIRAP.

Positive Control

Found in the cytoplasm and highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.

Product Categories/Family for anti-TIRAP antibody

Polyclonal Antibody; Chemokines & Receptors

Applications Tested/Suitable for anti-TIRAP antibody

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Other Notes

Small volumes of anti-TIRAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIRAP purchase

MBS395869 is an antibody targeting TIRAP. Also known as Toll Interleukin 1 receptor adaptor protein (TIRAP) (CT). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TIRAP. It may not necessarily be applicable to this product.

NCBI GI #

295390024

NCBI GeneID

117149

NCBI Accession #

NP_473437.2

UniProt Primary Accession #

Q99JY1

UniProt Secondary Accession #

Q91ZW0

UniProt Related Accession #

Q99JY1

GenBank Nucleotide #

NM_054096.2

NCBI Official Full Name

toll/interleukin-1 receptor domain-containing adapter protein

NCBI Official Synonym Full Names

toll-interleukin 1 receptor (TIR) domain-containing adaptor protein

NCBI Official Symbol

Tirap

NCBI Official Synonym Symbols

Mal; Wyatt; Tlr4ap; AA407980; C130027E04Rik

UniProt Protein Name

Toll/interleukin-1 receptor domain-containing adapter protein

UniProt Synonym Protein Names

Adaptor protein Wyatt; MyD88 adapter-like protein

Protein Family

Toll/interleukin-1 receptor domain-containing adapter protein

UniProt Gene Name

Tirap

UniProt Synonym Gene Names

Mal; TIR domain-containing adapter protein

UniProt Entry Name

TIRAP_MOUSE

NCBI Protein Information

toll/interleukin-1 receptor domain-containing adapter protein; adaptor protein Wyatt; myD88 adapter-like protein; Toll-like receptor 4 adaptor protein; TIR domain-containing adapter protein

UniProt Comments for TIRAP

Function: Adapter involved in the TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response

By similarity. Positively regulates the production of TNF-alpha and interleukin-6

By similarity.

Subunit structure: Homodimer. Also forms heterodimers with MYD88. Interacts with BMX and TBK1

By similarity. Interacts with TLR4 and IRAK2 via their respective TIR domains. Interacts with EIF2AK2. Does not interact with IRAK1-1, nor TLR9. May interact with PIK3AP1. Ref.4

Subcellular location: Cytoplasm

By similarity. Cell membrane

By similarity. Membrane

By similarity. Note: Colocalizes with DAB2IP at the plasma membrane

By similarity.

Post-translational modification: Phosphorylated by IRAK1 and IRAK4

By similarity. Also phosphorylated by BTK

By similarity.Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation

By similarity.

Sequence similarities: Contains 1 TIR domain.

Research Articles on TIRAP

1. TIRAP plays a central role in regulating the effects of lipopolysaccharides or lipoteichoic acid on chlorpromazine-induced hepatotoxicity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TIRAP antibody

Products by Pathway	Pathway Diagram
Hepatitis B Pathway antibodies	Hepatitis B Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
NF-kappa B Signaling Pathway antibodies	NF-kappa B Signaling Pathway Diagram
Pertussis Pathway antibodies	Pertussis Pathway Diagram
Pertussis Pathway antibodies	Pertussis Pathway Diagram
TLR4 (Toll-like Receptor 4) Signaling Pathway antibodies	TLR4 (Toll-like Receptor 4) Signaling Pathway Diagram
Toll Like Receptor 4 (TLR4) Cascade Pathway antibodies	Toll Like Receptor 4 (TLR4) Cascade Pathway Diagram
Toll Like Receptor Signaling Pathway antibodies	Toll Like Receptor Signaling Pathway Diagram
Toll-Like Receptors Cascades Pathway antibodies	Toll-Like Receptors Cascades Pathway Diagram

Diseases associated with anti-TIRAP antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>65 publications with TIRAP and Inflammation
Necrosis Antibodies	>32 publications with TIRAP and Necrosis
Immune System Diseases Antibodies	>24 publications with TIRAP and Immune System Diseases
Tuberculosis Antibodies	>22 publications with TIRAP and Tuberculosis
Malaria Antibodies	>14 publications with TIRAP and Malaria
Cardiovascular Diseases Antibodies	>11 publications with TIRAP and Cardiovascular Diseases
Neoplasms Antibodies	>10 publications with TIRAP and Neoplasms
Nervous System Diseases Antibodies	>8 publications with TIRAP and Nervous System Diseases
MYCOBACTERIUM TUBERCULOSIS, SUSCEPTIBILITY TO Antibodies	>7 publications with TIRAP and MYCOBACTERIUM TUBERCULOSIS, SUSCEPTIBILITY TO
Disease Models, Animal Antibodies	>4 publications with TIRAP and Disease Models, Animal

Organs/Tissues associated with anti-TIRAP antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>17 publications with TIRAP and Liver
Lung Antibodies	>17 publications with TIRAP and Lung
Brain Antibodies	>12 publications with TIRAP and Brain
Kidney Antibodies	>11 publications with TIRAP and Kidney
Bone Antibodies	>9 publications with TIRAP and Bone
Spleen Antibodies	>8 publications with TIRAP and Spleen
Heart Antibodies	>6 publications with TIRAP and Heart
Skin Antibodies	>6 publications with TIRAP and Skin
Intestine Antibodies	>6 publications with TIRAP and Intestine
Bone Marrow Antibodies	>6 publications with TIRAP and Bone Marrow