DRADA Antibody – Sheep Polyclonal

Function: Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Does not affect polyomavirus replication but provides protection against virus-induced cytopathic effects. Essential for embryonic development and cell survival and plays a critical role in the maintenance of hematopoietic stem cells

By similarity.

Subunit structure: Homodimer. Homodimerization is essential for its catalytic activity. Interacts with ILF2/NF45 and ILF3/NF90. Binding to ILF3/NF90 up-regulates ILF3-mediated gene expression. Interacts with UPF1 and EIF2AK2/PKR

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus › nucleolus

By similarity. Note: Shuttles between the cytoplasm and nucleus

By similarity.

Post-translational modification: Sumoylation reduces RNA-editing activity

By similarity.

Sequence similarities: Contains 1 A to I editase domain.Contains 2 DRADA repeats.Contains 3 DRBM (double-stranded RNA-binding) domains.