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CD54 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS397058

Monoclonal antibody 1304 detects CD54 (ICAM-1) and is useful for the detection of activated endothelial cells and other activated haematopoietic cells. The antibody inhibits the binding of natural killer (NK) cells to target cells (K562). Intercellular… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD54, Antibody
Full Product Name
CD54, ICAM-1
Product Gene Name
Isotype
IgG2a
Clone Number
1304
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Affinity Purified
Concentration
0.1 mg (lot specific)
Preparation and Storage
Store at 2-8 degree C.
Immunogen
Cultivated human endothelial cells
Reconstitution
Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, Phosphate Buffered Saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.
Positive Control
Human Tonsil
Incubation Time
60 min at RT or 18 hr at 2-8 degree C
Pre-Treatment
Formaldehyde-fixed tissue slices are preferably pre-treated with proteinase K.
Applications Tested/Suitable for anti-CD54 antibody
Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes for anti-CD54 antibody
IHC-P: 2 ug/ml

Product References and Citations for anti-CD54 antibody

1. Albelda S.M., and Buck C.A. (1990) Integrins and other cell adhesion molecules. FASEB J. 4; 2868-2880. 2. Freudenthal P.S., and Steinmann R.M. (1990) The distinct surface of human blood dendritic cells, as observed after an improved isolation method. PNAS 87; 7698-7702. 3. Klickstein L.B., and Springer T.A. (1995) CD54 (ICAM-1)cluster report. In Leucocyte Typing V: White Cell Differentiation Antigens. S. Schlossman et al. eds.Oxford University Press pp.1548-1550.

Other Notes

Small volumes of anti-CD54 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD54 purchase

MBS397058 is an antibody targeting CD54. Also known as CD54, ICAM-1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD54. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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