Rabbit Anti-COL4A1 Antibody – Cat# MBS397133

Collagen Type IV (COL4A1) Antibody – Rabbit

Name: COL4A1 Antibody; Rabbit; Pig-Reactive; Pig-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS397133
Price: 690 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS397133

Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Collagen Type IV (COL4A1), Antibody

Full Product Name

Collagen Type IV, bovine

Product Gene Name

anti-COL4A1 antibody

Host

Rabbit

Species Reactivity

Cattle, Pig.
Other species < 0.1% cross-reaction

Form/Format

Lyophilized from phosphate buffered solution; no BSA and preservative added.

Purity/Purification

Affinity Purified

Concentration

1 mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C.

Molecular Weight

160,412 Da

3D Structure

ModBase 3D Structure for Q7SIB2

Immunogen

Purified collagen type IV from bovine lens capsule

Positive Control

Bovine skin or lens capsule

Incubation Time

IHC(P) 60 min at RT or 2-8 degree C over night

Pre-Treatment

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications Tested/Suitable for anti-COL4A1 antibody

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA), Immunoblotting (IB), Western Blot (WB)

Application Notes for anti-COL4A1 antibody

IF: 1:40-1:80
IHC-P: 1:500-1:1000
ELISA: 1:200

Product References and Citations for anti-COL4A1 antibody

1. Guerret S, Govignon E, Hartmann DJ, Ronfard V. (2003) Long-term remodeling of a bilayered living human skin equivalent (Apligraf) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Repair Regen. Jan-Feb;11(1):35-45. 2. Vitellaro-Zuccarello L., Garbelli R., Dal Pozzo Rossi V. (1992) Immunocytochemical localization of collagen types I, III, IV, and fibronectin in the human dermis. Cell Tissue Res. 268, 505-511. 3. Lannes-Vieira J., Dardenne M., Savino W. (1991) Extracellular matrix components of the mouse thymus microenvironment: Ontogenetic studies and modulation by glucocorticoi•d hormones. J. Histochem. Cytochem. 39, 1539-1546.

Other Notes

Small volumes of anti-COL4A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COL4A1 purchase

MBS397133 is an antibody targeting COL4A1. Also known as Collagen Type IV, bovine. Reactive in Cattle, Pig.
Other species < 0.1% cross-reaction. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for COL4A1. It may not necessarily be applicable to this product.

NCBI GI #

1270532995

NCBI Accession #

Q7SIB2.2

UniProt Primary Accession #

Q7SIB2

UniProt Secondary Accession #

G1K238

NCBI Official Full Name

Collagen alpha-1(IV) chain

UniProt Protein Name

Collagen alpha-1(IV) chain

Protein Family

Collagen

UniProt Gene Name

COL4A1

UniProt Comments for COL4A1

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.