Rabbit Anti-COL3A1 Antibody – Cat# MBS397143

Procollagen Type III (COL3A1) Antibody – Rabbit

Name: COL3A1 Antibody; Rabbit; Human-Reactive; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS397143
Price: 730 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS397143

Collagen type III is synthesized as a homo-trimeric pro-collagen comprising three identical pro-alpha(III)-chains. It has been reported that procollagen type III is processed extracellularly at the ECM and can be found by immunostaining intracellular as well… More details

$730 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Procollagen Type III (COL3A1), Antibody

Full Product Name

Procollagen Type III, human, bovine

Product Gene Name

anti-COL3A1 antibody

Host

Rabbit

Species Reactivity

Human, Cattle

Form/Format

Lyophilized from phosphate buffered solution; no BSA and preservative added.

Purity/Purification

Affinity Purified

Concentration

1 mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C.

Molecular Weight

111,907 Da

3D Structure

ModBase 3D Structure for P02461

Immunogen

Procollagen type III deprived of the C-terminal PIIICP peptide

Positive Control

Human or bovine skin and liver

Incubation Time

IHC(P) 60 min at RT or 2-8 degree C over night

Pre-Treatment

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Applications Tested/Suitable for anti-COL3A1 antibody

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA), Immunoblotting (IB), Western Blot (WB)

Application Notes for anti-COL3A1 antibody

IF: 1:40
IHC-P: 1:500
ELISA: 1:100-1:200
RIA: >1:200

Product References and Citations for anti-COL3A1 antibody

1. Desmoulière A., Darby I., Monte Alto Costa A., Raccurt M., Tuchweber B., Sommer P., Gabbiani G. (1997) Extracellular Matrix Deposition, lysyl oxidase expression, and myofibroblastic differentiation during the initial stages of cholestatic fibrosis in the rat. Lab. Invest. 76, 765-778. 2. Gindre D., Peyrol S., Raccurt M., Sommer P., Loire. R., Grimaud J.A., Cordier J.F. (1995) Fibrosing vasculitis in Wegener's granulomatosis: ultrastructural and immunohistochemical analysis of the vascular lesions. Virchows Arch. 427, 385-393. 3. Trinchet J.C., Hartmann D.J., Pateron D., Munz-Gotheil C., Callard P., Ville G., Beaugrand M. (1992) Serum type I collagen and N-terminal peptide of type III procollagen in patients with alcoholic liver disease: relationship to liver histology. Alcohol. Clin. Exp. Res. 16, 342-346.

Other Notes

Small volumes of anti-COL3A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COL3A1 purchase

MBS397143 is an antibody targeting COL3A1. Also known as Procollagen Type III, human, bovine. Reactive in Human, Cattle. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for COL3A1. It may not necessarily be applicable to this product.

NCBI GI #

4502951

NCBI GeneID

1281

NCBI Accession #

NP_000081.1

UniProt Primary Accession #

P02461

UniProt Secondary Accession #

P78429; Q15112; Q16403; Q53S91; Q541P8; Q6LDB3; Q6LDJ2; Q6LDJ3; Q7KZ56; D2JYH5; D3DPH4

UniProt Related Accession #

P02461

GenBank Nucleotide #

NM_000090.3

NCBI Official Full Name

collagen alpha-1(III) chain preproprotein

NCBI Official Synonym Full Names

collagen type III alpha 1 chain

NCBI Official Symbol

COL3A1

NCBI Official Synonym Symbols

EDS4A; EDSVASC

UniProt Protein Name

Collagen alpha-1(III) chain

Protein Family

Collagen

UniProt Gene Name

COL3A1

NCBI Protein Information

collagen alpha-1(III) chain

NCBI Summary for COL3A1

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

UniProt Comments for COL3A1

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Research Articles on COL3A1

1. Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-COL3A1 antibody

Reference Product	PubMed Publications
SPARC antibody	>18 publications with COL3A1 and SPARC
THBS1 antibody	>3 publications with COL3A1 and THBS1
HTRA1 antibody	>1 publications with COL3A1 and HTRA1
DDR1 antibody	>1 publications with COL3A1 and DDR1

Pathways associated with anti-COL3A1 antibody

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Biosynthesis And Modifying Enzymes Pathway antibodies	Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Endothelins Pathway antibodies	Endothelins Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram

Diseases associated with anti-COL3A1 antibody

Disease Name	Pubmed Publications
Fibrosis Antibodies	>56 publications with COL3A1 and Fibrosis
Heart Diseases Antibodies	>26 publications with COL3A1 and Heart Diseases
Inflammation Antibodies	>21 publications with COL3A1 and Inflammation
Neoplasms Antibodies	>19 publications with COL3A1 and Neoplasms
Hypertension Antibodies	>10 publications with COL3A1 and Hypertension
Hypertrophy Antibodies	>8 publications with COL3A1 and Hypertrophy
Liver Diseases Antibodies	>8 publications with COL3A1 and Liver Diseases
Kidney Diseases Antibodies	>8 publications with COL3A1 and Kidney Diseases
Myocardial Infarction Antibodies	>6 publications with COL3A1 and Myocardial Infarction
Hyperplasia Antibodies	>6 publications with COL3A1 and Hyperplasia

Organs/Tissues associated with anti-COL3A1 antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>115 publications with COL3A1 and Vascular
Connective Tissue Antibodies	>94 publications with COL3A1 and Connective Tissue
Skin Antibodies	>88 publications with COL3A1 and Skin
Blood Antibodies	>68 publications with COL3A1 and Blood
Muscle Antibodies	>63 publications with COL3A1 and Muscle
Bone Antibodies	>39 publications with COL3A1 and Bone
Heart Antibodies	>30 publications with COL3A1 and Heart
Lung Antibodies	>23 publications with COL3A1 and Lung
Uterus Antibodies	>15 publications with COL3A1 and Uterus
Kidney Antibodies	>15 publications with COL3A1 and Kidney