HEA125 Antibody – Mouse

The antibody HEA 125 reacts intensely with all carcinoma derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate and metastases thereof. Stratified squamous epithelia usually stain less intensely then adenocarcinoma. The antibody is suitable for differentiation between carcinoma an non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained as sarcoma, lymphoma, melanoma and neurogenic tumours do. Epithelial cells express a number of characteristic proteins and glycoproteins like cytokeratins, epithelial membrane antigen and epithelium-specific glycoprotein Egp34 (HEA125). The monoclonal antibody HEA125 which has been derived from immunisation of mice with a colon carcinoma cell line shows a broad reactivity pattern. It is labelling most human epithelial cells and epithelium derived tumours. It doesn't react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues. 34 kDa Epithelium-specific membrane glycoprotein HEA 125.

1. Moldenhauer G., Momburg F., Möller P., Schwartz, and Hämmerling G.J. (1987) Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer 56; 714-721. 2. Momburg F., Moldenhauer G., Hämmerling G.J., and Möller F. (1987) Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Research 47; 2883-2891. 3. Simon B., Podolsky, D.K., Moldenhauer G., Isselbacher K.J., Gattoni-Celli, S., and Brand S.J. (1990) Epithelial glykoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc. Natl., Acad. Sci. USA 87; 2755 ff. 4. Kemmner W., Moldenhauer G., Schlag P., and Brossmer R. (1992) Separation of tumor cells from a suspension of dissociated human colorectal carcionoma tissue by means of monoclonal antibody-coated magnetic beads. J. Immunol. Methods 147; 197-200. 5. Winter, M. J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am. J. Pathol. 163: 2139-2148.

Small volumes of anti-HEA125 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS397229 is an antibody targeting HEA125. Also known as HEA125, Epithelium-specific Glycoprotein, Egp34, EpCAM (CD326). Reactive in Human. Host: Mouse.