25(oh)vitamin D3-1-Alpha-Hydroxylase Antibody – Sheep

The polyclonal antibody is well suited for the detection of 25(Hydroxy)Vitamin D3-1-alpha-Hydroxylase in tissues and tissue extracts e.g. in HKC-8 cell line from human proximal tubules (1). The seco-steroid hormone 1,25-Dihydroxyvitamin D3 plays a vital role in Calcium metabolism, in tissue differentiation and during the growth of bone. The biosynthesis of the hormone is catalysed by a mitochondrial P450-enzyme, 25-Hydroxy-Vitamin D3 1-alpha-Hydroxylase. Although the enzyme has been detected in different tissues, the main site of Vitamin D3 synthesis is the kidney. 25(OH)Vitamin D3-1-alpha-Hydroxylase, 56 kDa Protein in Western Blot.

1. Bland R, Walker EA, Hughes SV, Stewart PM, Hewison M. (1999) Constitutive expression of 25-hydroxyvitamin D3-1-alpha-hydroxylase in a transformed human proximal tubule cell line: Evidence for direct regulation of vitamin D metabolism by calcium. Endocrinology 140(5), 2027-2034. 2. Zehnder D, Bland R, Walker EA, Bradwell AR, Howie AJ, Hewison M, Stewart PM. (1999) Expression of 25-Hydroxyvitamin D3-1-alpha-hydoxylase in human kidney. J. Am. Soc. Nephrol. 10; 2465-2473 3. Bland R, Markovic D, Hills CE, Hughes SV, Chan SL, Squires PE, Hewison M (2004) Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in pancreatic islets. J Steroid Biochem Mol Biol. 89-90(1-5):121-125. 4. Takeyama K, Kitanaka S, Sato T, Kobori M, Yanagisawa J, Kato S. (1997) 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science;277(5333):1827-30 (cloning of 1-alpha-Hydroxylase).

Small volumes of anti-25(OH)Vitamin D3-1-alpha-Hydroxylase antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS397242 is an antibody targeting 25(OH)Vitamin D3-1-alpha-Hydroxylase. Reactive in Mouse, Human. Host: Sheep.