Mouse Anti-Ki-67 Antibody – Cat# MBS397261

Ki-67 Antibody – Mouse

Name: Ki-67 Antibody; Mouse; Human-Reactive; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS397261
Price: 730 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS397261

In retrospective studies the intensity of staining and the frequency of Ki-67 expression in cell nuclei was interpreted as a prognostic factor for the malignancy of tumours (Gerdes et al., 1987). Ki-67 Protein is a nuclear protein, which is ubiquitous present… More details

$730 / 5 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ki-67, Antibody

Full Product Name

Ki-67, Cell Cycle Marker (MIB-1)

Product Gene Name

anti-Ki-67 antibody

Isotype

IgG1

Clone Number

MIB-1

Host

Mouse

Species Reactivity

Human

Form/Format

Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

324,718 Da

3D Structure

ModBase 3D Structure for Q61769

Immunogen

Human nuclear Ki-67 antigen

Positive Control

Breast or colon carcinoma

Incubation Time

60 min at RT

Pre-Treatment

Unmasking fluid G () or C at 90-100 degree C

Applications Tested/Suitable for anti-Ki-67 antibody

Immunohistochemistry (IHC) Formalin/Paraffin

Application Notes for anti-Ki-67 antibody

Working Concentration: (RTU) neat

Product References and Citations for anti-Ki-67 antibody

1. Gerdes J., Schwab U., Lemke H., and Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int. J. Cancer 31; 13-20. 2. Gerdes J., Stein H., Pileri S., Rivano M.T., Gobbi M., Ralfkiaer E., Nielsen K.M., Pallesen G., Bartels H., Palestro G., and Delsol G. (1987) Prognostic relevance of the tumour-cell growth fraction in malignant non-Hodgkin's lymphomas. Letter to the editor: Lancet ii, 448-449. 3. Gerdes J., Becker M.H.G., Key G., and Cattoretti G. (1992) Immunohistochemical detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J. Pathol., 168; 85-86. 4. Kubbutat M.H.G., Cattoretti G., Gerdes J., Key G., (1994) Comparison of monoclonal antibodies PC10 and MIB-1 on microwave-processed paraffin sections. Cell Proliferation 27; 553-559.

Other Notes

Small volumes of anti-Ki-67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ki-67 purchase

MBS397261 is an antibody targeting Ki-67. Also known as Ki-67, Cell Cycle Marker (MIB-1). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Ki-67. It may not necessarily be applicable to this product.

NCBI GI #

1177528

NCBI GeneID

17345

NCBI Accession #

CAA58026.1

UniProt Primary Accession #

Q61769

UniProt Secondary Accession #

Q61769; Q7TSF6

UniProt Related Accession #

E9PVX6

NCBI Official Full Name

Ki-67

NCBI Official Synonym Full Names

antigen identified by monoclonal antibody Ki 67

NCBI Official Symbol

Mki67

NCBI Official Synonym Symbols

Ki67; Ki-67; D630048A14Rik

UniProt Protein Name

Proliferation marker protein Ki-67

UniProt Synonym Protein Names

Antigen identified by monoclonal antibody Ki-67 homologCurated; Antigen KI-67 homologCurated; Antigen Ki67 homologCurated

UniProt Gene Name

Mki67

UniProt Synonym Gene Names

Antigen KI-67 homologCurated; Antigen Ki67 homologCurated

NCBI Protein Information

proliferation marker protein Ki-67; antigen KI-67

UniProt Comments for Ki-67

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.

Research Articles on Ki-67

1. injection of a b3-adrenergic receptor (b3-AR) agonist for continuous 5 days increased the number of Ki67-positive brown adipocytes even at Day 1 but not that of SV cells. In addition, the b3-AR antagonist, but not b1-AR antagonist, attenuated the cold exposure-induced increase in the number of Ki67-positive brown adipocytes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-Ki-67 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1510 publications with Ki-67 and Brain
Skin Antibodies	>1007 publications with Ki-67 and Skin
Lung Antibodies	>792 publications with Ki-67 and Lung
Prostate Antibodies	>545 publications with Ki-67 and Prostate
Intestine Antibodies	>468 publications with Ki-67 and Intestine
Kidney Antibodies	>422 publications with Ki-67 and Kidney
Eye Antibodies	>330 publications with Ki-67 and Eye
Bone Marrow Antibodies	>298 publications with Ki-67 and Bone Marrow
Embryonic Tissue Antibodies	>282 publications with Ki-67 and Embryonic Tissue
Thyroid Antibodies	>265 publications with Ki-67 and Thyroid