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Green Fluorescent (GFP) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS397396

Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited… More details

$725 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Green Fluorescent (GFP), Polyclonal Antibody
Full Product Name
Rabbit anti-native Green Fluorescent Protein (Immunoaffinity purified)
Product Gene Name
anti-GFP antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Aequoria Victoria (Native) GFP, Recombinant (E.Coli) and Native GFP-Fusion Proteins, and All Variants of GFP tested.
Form/Format
10 mM TRIS buffer pH 8.0, containing 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Specificity
The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.
Preparation and Storage
The antibody is shipped at ambient temperature and may be stored at +4 degree C. For prolonged storage prepare appropriate aliquots and store at or below -20 degree C.
Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2).
Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Molecular Weight
26,886 Da
3D Structure
ModBase 3D Structure for P42212
Source Note
The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish.
Applications Tested/Suitable for anti-GFP antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes for anti-GFP antibody
ELISA: 1:5,000 to 1:25,000
Immunoblotting: 1:2,000 to 1:10,000
Immunocytochemistry the antiserum can be diluted 1:100 to 1:250.

Product References and Citations for anti-GFP antibody

1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.

Other Notes

Small volumes of anti-GFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GFP purchase

MBS397396 is an antibody targeting GFP. Also known as Rabbit anti-native Green Fluorescent Protein (Immunoaffinity purified). Reactive in Aequoria Victoria (Native) GFP, Recombinant (E.Coli) and Native GFP-Fusion Proteins, and All Variants of GFP tested.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GFP. It may not necessarily be applicable to this product.

NCBI GI #
1169893
NCBI Accession #
P42212.1
UniProt Primary Accession #
P42212
UniProt Secondary Accession #
Q17104; Q27903; Q93125
NCBI Official Full Name
Green fluorescent protein
UniProt Protein Name
Green fluorescent protein
Protein Family
Green fluorescent protein
UniProt Gene Name
GFP

UniProt Comments for GFP

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GFP antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with GFP and FLNA

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