COVID 19 Nucleocapsid (Np) Total Antibody Coronavirus (COVID-19) ELISA Kit for Human

Intended Uses: SARS-CoV-2 NP Ab ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the detection and qualitative measurement of total antibodies against the nucleocapsid protein (NP) of SARS-CoV-2 virus in human blood.

Principle of the Assay: SARS-CoV-2 NP Ab ELISA kit is a two-step incubation immunoassay kit. Recombinant nucleocapsid protein (NP) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-NP antibodies in human serum or plasma specimens. After a 1-hour incubation, anti-NP antibodies are captured by immobilized NP protein while the unbound components are washed away. Afterwards, a detection solution containing HRP- conjugated NP is added for another 1-hour incubation, wherein HRP-conjugated NP binds to the total antibodies previously bound to NP protein on the plate. After removal of nonspecific bindings, a HRP substrate solution containing 3,3?,5,5?-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow signals, which is quantified by an absorbance microplate reader at 450nm. The color intensity is proportional to the amount of anti-NP total antibodies captured inside the wells.

Small volumes of COVID-19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS398007 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COVID 19 Nucleocapsid (NP) total antibody Coronavirus (COVID-19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COVID-19. The ELISA analytical biochemical technique of the MBS398007 kit is based on COVID-19 antibody-COVID-19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COVID-19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COVID-19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).