APPL1 Antibody – Rabbit Polyclonal

The adaptor proteins APPL1 and APPL2 (Adaptor protein-containing an NH2-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central Pleckstrin homology (PH) domain, Phosphotyrosine-binding (PTB) domain, and Leucine zipper motif), regulate adiponectin action and insulin-sensitivity in multiple insulin-responsive tissues.
APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR)domain, a central pleckstrin homology (PH) domain and a COOH-terminalphosphotyrosine binding (PTB) domain [1], was originally identified as an interactingpartner of Akt in a yeast two-hybrid assay using Akt2 as a bait [2]. APPL1 binds to anumber of cell surface receptors (TrkA[3, 4], DCC[5], adiponectin [6, 7], FSH[8]) andintracellular signaling molecules (small GTPase Rab5[9], GIPC[4] and inositol 5-phosphatase[10], suggesting that APPL1 may act as a common relay to coordinate diversesignaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteractingthe effect of the Akt inhibitor TRB3 [11].

1. Signaling mechanisms underlying the insulin-sensitizing effects of adiponectin. Cheng KK, Lam KS, Wang B, Xu A. Best Pract Res Clin Endocrinol Metab. 2014 28(1):3-13
2. APPL1: role in adiponectin signaling and beyond. Deepa SS, Dong LQ. Am J Physiol Endocrinol Metab. 2009 296(1):E22-36

1. Hosch, S.E., J.M. Olefsky, and J.J. Kim, APPLied mechanics: uncovering howadiponectin modulates insulin action. Cell Metab, 2006. 4(1): p. 5-6.
2. Mitsuuchi, Y., et al., Identification of a chromosome 3p14.3-21.1 gene, APPL,encoding an adaptor molecule that interacts with the oncoproteinserine/threonine kinase AKT2. Oncogene., 1999. 18(35): p. 4891-8.
3. Lin, D.C., et al., APPL1 associates with TrkA and GIPC1, and is required forNGF-mediated signal transduction. Mol Cell Biol, 2006. 25: p. 25.
4. Varsano, T., et al., GIPC is recruited by APPL to peripheral TrkA endosomes andregulates TrkA trafficking and signaling. Mol Cell Biol, 2006. 26(23): p. 8942-52.
5. Liu, J., et al., Mediation of the DCC apoptotic signal by DIP13 alpha. J BiolChem., 2002. 277(29): p. 26281-5. Epub 2002 May 14.
6. Cheng, K.K., et al., Adiponectin-induced endothelial nitric oxide synthaseactivation and nitric oxide production are mediated by APPL1 in endothelial cells.Diabetes, 2007. 56(5): p. 1387-94.
7. Mao, X., et al., APPL1 binds to adiponectin receptors and mediates adiponectinsignalling and function. Nat Cell Biol., 2006. 8(5): p. 516-23. Epub 2006 Apr 16.
8. Nechamen, C.A., et al., Human follicle-stimulating hormone (FSH) receptorinteracts with the adaptor protein APPL1 in HEK 293 cells: potential involvementof the PI3K pathway in FSH signaling. Biol Reprod., 2004. 71(2): p. 629-36.Epub 2004 Apr 7.
9. Miaczynska, M., et al., APPL proteins link Rab5 to nuclear signal transductionvia an endosomal compartment. Cell., 2004. 116(3): p. 445-56.
10. Erdmann, K.S., et al., A role of the Lowe syndrome protein OCRL in early steps ofthe endocytic pathway. Dev Cell, 2007. 13(3): p. 377-90.
11. Cheng, K.K., et al., APPL1 potentiates insulin-mediated inhibition of hepaticglucose production and alleviates diabetes via Akt activation in mice. Cell Metab,2009. 9(5): p. 417-27.

Small volumes of anti-APPL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS398023 is an antibody targeting APPL1. Also known as Adaptor Protein, Phosphotyrosine Interacting with PH Domain And Leucine Zipper 1 (Appl1): Human, rabbit polyclonal Antibody. Reactive in Human. Host: Rabbit.