Fibroblast Growth Factor 21 (FGF-21) (FGF21) Recombinant Protein

Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic beta-cells. FGF- 21 stimulates glucose uptake in adipocytes. It also protects animals from diet- induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance. Adiponectin has been shown to be critical in mediating the beneficial metabolic actions of FGF-21.
FGF21, a polypeptide with 210 amino acid residues produced mostly from the liver tissue[1]. Mouse FGF21 shares 75% identity as human FGF21. Recent animal studiesindicate it possesses potent beneficial effects on glucose and lipid metabolism andinsulin sensitivity.[2] Increasing data shows FGF21 can significantly stimulateglucose uptake in mature adipocytes. And The lowered LDL-cholesterol and increasedHDL-cholesterol can also be observed[2,3]. FGF21 exerts its bioactivity throughinteraction with membrane bound FGF receptors (FGFRs) which requires beta-Klotho asa co-factor to bind and activate FGFR signaling[4,5]. The activation of FGF21 caninduce the stimulation of diverse downstream pathways medicated by MAPK,FRS-2,SHP-2, PI3K, raf, stat and other signaling molecules[6-9]. In sum, FGF21 induces avariety of significant beneficial metabolic changes without apparent adverse effectswhich makes this factor a hot candidate to treat some metabolic diseases[10].
Total 207AA Mw: 23kDa (calculated). N-terminal His-tag and TEV cleavage site, 25 extra AA.

1. Stressed Liver and Muscle Call on Adipocytes with FGF21. Luo Y, McKeehan WL. Front Endocrinol 2013 18;4:194
2. Fibroblast growth factor 21 as an emerging metabolic regulator: clinical perspectives. Woo YC, Xu A, Wang Y, Lam KS. Clin Endocrinol (Oxf). 2013 Apr;78(4):489-96

[1] Nishimura T, Nakatake Y, Konishi M, Itoh N: Identification of a novel FGF, FGF -21, preferentially expressed inthe liver. Biochim Biophys Acta1492:203-206, 2000
[2] Kharitonenkov A et al. FGF-21 as a novel metabolic regulator. J Clin Invest 115:1627-1635, 2005
[3]Alexei Kharitonenkov et al. The Metabolic State of Diabetic Monkeys Is Regulated by Fibroblast Growth Factor21.Endocrinology.148(2):774-781. 2007
[4] Hiroshi Kurosu et al. Tissue-speci c Expression of betaKlotho and Fibroblast Growth Factor (FGF) Receptor IsoformsDetermines Metabolic Activity of FGF19 and FGF21. J Biol Chem. 282(37): 26687-26695. 2007
[5] Ogawas Y et al. BetaKlotho is required for metabolic activity of fibroblast growth factor 21 Proc Natl Acad SciUSA 104: 7432-7437, 2007.
[6]Steven L. PELECH et al. Fibroblast growth factor treatment of Swiss 3T3 cells activates asubunit S6 kinase that phosphorylates a synthetic peptide substrate. Proc. Natl. Acad. Sci. USAVol. 83, pp. 5968-5972, August 1986
[7]Rosa Carballada et al. Phosphatidylinositol-3 kinase acts in parallel to the ERK MAP kinase in the FGF pathwayduring Xenopus mesoderm induction. Development 128, 35-44 (2001)
[8]Dayanand D. Deo et al. Phosphorylation of STAT-3 in Response to Basic Fibroblast GrowthFactor Occurs through a Mechanism Involving Platelet-activating Factor, JAK-2, and Src in Human Umbilical VeinEndothelial Cells. The journal of molecular chemistry. Vol. 277, No. 24, Issue of June 14, pp. 21237-21245, 2002
[9]Maria I. Kontaridis et al. Role of SHP-2 in Fibroblast Growth Factor Receptor-MediatedSuppression of Myogenesis in C2C12 Myoblasts. Molecular and cellular biology, Vol. 22, No. 11, p. 3875-3891. 2002
[10] Dostálová I, Haluzíková D, Haluzík M.Fibroblast Growth Factor 21: A Novel Metabolic Regulator With PotentialTherapeutic Properties in Obesity/Type 2 Diabetes Mellitus. Physiol. Res. 58: 1-7, 2009

Small volumes of FGF21 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS398087 is a recombinant protein targeting FGF21. Also known as Fibroblast growth factor 21 (FGF-21): Mouse, His-tagged (Recombinant protein). Host: E Coli.