Procollagen Type 1 N-Terminal Pro (P1NP) ELISA Kit for Human

Background: In bone, collagen type I is synthesized by osteoblasts in the form of procollagen. This precursor contains a short signal sequence and terminal extension peptides: amino-terminal propeptide (PINP) and carboxy-terminal propeptide. These propeptide extensions are removed by specific proteinases before the collagen molecules form. Both propeptides can be found in the circulation and their concentration reflects the synthesis rate of collagen type I [1] [2].

Principle of the Assay: This assay is a quantitative sandwich ELISA. The immunoplate is precoated with a monoclonal antibody specific for human P1NP. Standards and samples are pipetted into the wells and any human P1NP present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human P1NP is added to the wells. After wash step to remove any unbound reagents, streptavidinHRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human P1NP bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human P1NP, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Intended Uses: This human P1NP ELISA kit is designed for quantification of human P1NP in serum, plasma and cell culture supernate samples.

[1] Patrick R. L., et al. (2012) Cold Spring Harb Perspect Med 2: a 00627.
[2] Morikawa N., et al. (2019) Int Heart J 20;60(2):310-317.

Small volumes of P1NP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS398102 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Procollagen type 1 N-terminal propeptide (P1NP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing P1NP. The ELISA analytical biochemical technique of the MBS398102 kit is based on P1NP antibody-P1NP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P1NP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P1NP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).