Proinsulin ELISA Kit for Human

Background: Proinsulin is a polypeptide of 86 amino acids made in the beta cells of the pancreas and is the precursor molecule for insulin, most proinsulin is converted to insulin and C-peptide secreted into the blood. Increased circulating levels of proinsulin are found in patients with insulinomas, hypoglycemia and hyperinsulinema.

Principle of the Assay: This assay is a quantitative sandwich ELISA. The immunoplate is precoated with a monoclonal antibody specific for human proinsulin. Standards and samples are pipetted into the wells and any human proinsulin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled monoclonal antibody specific for human proinsulin is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human proinsulin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human proinsulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Intended Uses: This human proinsulin ELISA kit is designed for quantification of human proinsulin in serum and plasma samples.

Small volumes of Proinsulin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS398104 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proinsulin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Proinsulin. The ELISA analytical biochemical technique of the MBS398104 kit is based on Proinsulin antibody-Proinsulin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Proinsulin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Proinsulin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).