Ovine oplGH Active Protein – Cat# MBS400051

Placental Growth Hormone (oplGH) Ovine Active Protein

Name: oplGH Active Protein; Placental Growth Hormone; Ovine; MyBioSource
Brand: MyBioSource
SKU: MBS400051
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400051

Recombinant ovine GH-22K produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 21980 Dalton. Predicted pI=7.81. oGH is purified by proprietary chromatographic techniques. It differs from… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

placental growth hormone (oplGH), Active Protein

Full Product Name

Ovine placental growth hormone

Product Gene Name

oplGH active protein

Product Synonym Names

Ovine placental growth hormone (oplGH)

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Ph e-Pro-Ala, Less than 4% as determined by gel filtration chromatogr aphy

Host

E Coli

Form/Format

Lyophylized white powder

Purity/Purification

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Preparation and Storage

Lyophilized oplGH although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization oGH can be stored at 4 degree C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Molecular Weight

27,101 Da

OMIM

139240

3D Structure

ModBase 3D Structure for P01242

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized oGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.

Protein Content

GH.Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.684 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH-22K-pl as a Reference Standard.

Dimers and Aggregates

Less than 4% as determined by gel filtration chromatography

Endotoxin

Less than 0.1 ng/ug (IEU/ug) of oplGH (22 K).

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 9.

Biological Activity

oplGH is fully biologically active when compared to World Health Organization (WHO) reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).

Product Categories/Family for oplGH active protein

Growth Hormones; prolactins; placental lactogens

Other Notes

Small volumes of oplGH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for oplGH purchase

MBS400051 is an active protein targeting oplGH. Also known as Ovine placental growth hormone. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for oplGH. It may not necessarily be applicable to this product.

NCBI GI #

2459884

NCBI GeneID

2689

NCBI Accession #

AAB71829.1

UniProt Primary Accession #

P01242

UniProt Secondary Accession #

O14643; O14644; P09587; B1A4H5; B1A4H7

UniProt Related Accession #

P01242

NCBI Official Full Name

placental growth hormone isoform hGH-V3

NCBI Official Synonym Full Names

growth hormone 2

NCBI Official Symbol

GH2

NCBI Official Synonym Symbols

GHL; GHV; GH-V; hGH-V

UniProt Protein Name

Growth hormone variant

UniProt Synonym Protein Names

Growth hormone 2; Placenta-specific growth hormone

UniProt Gene Name

GH2

UniProt Synonym Gene Names

GH-V

UniProt Entry Name

SOM2_HUMAN

NCBI Protein Information

growth hormone variant; placenta-specific growth hormone; placental-specific growth hormone

NCBI Summary for oplGH

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. As in the case of its pituitary counterpart, growth hormone 1, the predominant isoform of this particular family member shows similar somatogenic activity, with reduced lactogenic activity. Mutations in this gene lead to placental growth hormone/lactogen deficiency. [provided by RefSeq, Jul 2008]

UniProt Comments for oplGH

GH2: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular region

Molecular Function: hormone activity

Research Articles on oplGH

1. genetic association studies in populations in Estonia, Czech Republic, and Bashkortostan/Russia: Data suggest an SNP in GH2 (rs2006123) is associated with adult height (not body mass index); A-allele is associated with taller stature. [META-ANALYSIS]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with oplGH active protein

Reference Product	PubMed Publications
GHR active protein	>2 publications with oplGH and GHR
JAK2 active protein	>1 publications with oplGH and JAK2
STAT5B active protein	>1 publications with oplGH and STAT5B
PRLR active protein	>1 publications with oplGH and PRLR

Pathways associated with oplGH active protein

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Growth Hormone Receptor Signaling Pathway antibodies	Growth Hormone Receptor Signaling Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Nuclear Signaling By ERBB4 Pathway antibodies	Nuclear Signaling By ERBB4 Pathway Diagram

Diseases associated with oplGH active protein

Disease Name	Pubmed Publications
Silver-Russell Syndrome Antibodies	>1 publications with oplGH and Silver-Russell Syndrome

Organs/Tissues associated with oplGH active protein

Organ/Tissue Name	Pubmed Publications
Placenta Antibodies	>49 publications with oplGH and Placenta
Pituitary Gland Antibodies	>15 publications with oplGH and Pituitary Gland
Embryonic Tissue Antibodies	>9 publications with oplGH and Embryonic Tissue
Vascular Antibodies	>8 publications with oplGH and Vascular