Human VEGF Active Protein – Cat# MBS142118

Vascular Endothelial Growth Factor (VEGF) Human Active Protein

Name: VEGF Active Protein; Vascular Endothelial Growth Factor; Human; MyBioSource
Brand: MyBioSource
SKU: MBS142118
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS142118

Description: Vascular Endothelial Growth Factor Human Recombinant produced in E Coli is a double, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 38231 Dalton.The VEGF is purified by proprietary chromatographic… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Vascular Endothelial Growth Factor (VEGF), Active Protein

Full Product Name

Recombinant Human Vascular Endothelial Growth Factor

Product Gene Name

VEGF active protein

Product Synonym Names

VEGF Human; Vascular Endothelial Growth Factor Human Recombinant; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF; MGC70609

Sequence

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Host

E Coli

Form/Format

The VEGF protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution VEGF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

34,406 Da

OMIM

192240

3D Structure

ModBase 3D Structure for P15692

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Protein Content

VEGF protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard.

Biological Activity

VEGF activity was determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-8ng/ml, corresponding to a Specific Activity of 125,000-1,000,000IU/mg.

Product Categories/Family for VEGF active protein

Cytokines and Growth Factors; Growth Factors; VEGF

Other Notes

Small volumes of VEGF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VEGF purchase

MBS142118 is an active protein targeting VEGF. Also known as Recombinant Human Vascular Endothelial Growth Factor. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for VEGF. It may not necessarily be applicable to this product.

NCBI GI #

76781480

NCBI GeneID

7422

NCBI Accession #

NP_001020537.2

UniProt Primary Accession #

P15692

UniProt Secondary Accession #

O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7

UniProt Related Accession #

P15692

GenBank Nucleotide #

NM_001025366.2

NCBI Official Full Name

vascular endothelial growth factor A isoform a

NCBI Official Synonym Full Names

vascular endothelial growth factor A

NCBI Official Symbol

VEGFA

NCBI Official Synonym Symbols

VPF; VEGF; MVCD1

UniProt Protein Name

Vascular endothelial growth factor A

UniProt Synonym Protein Names

Vascular permeability factor; VPF

Protein Family

Vascular endothelial growth factor

UniProt Gene Name

VEGFA

UniProt Synonym Gene Names

VEGF; VEGF-A; VPF

UniProt Entry Name

VEGFA_HUMAN

NCBI Protein Information

vascular endothelial growth factor A; vascular permeability factor

NCBI Summary for VEGF

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for VEGF

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Research Articles on VEGF

1. High VEGF-A expression is associated with drug resistance in colorectal carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with VEGF active protein

Products by Pathway	Pathway Diagram
Angiogenesis Pathway antibodies	Angiogenesis Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Cellular Response To Hypoxia Pathway antibodies	Cellular Response To Hypoxia Pathway Diagram
Cellular Responses To Stress Pathway antibodies	Cellular Responses To Stress Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
EPH-Ephrin Signaling Pathway antibodies	EPH-Ephrin Signaling Pathway Diagram

Diseases associated with VEGF active protein

Disease Name	Pubmed Publications
Neovascularization, Pathologic Antibodies	>1888 publications with VEGF and Neovascularization, Pathologic
Retinal Diseases Antibodies	>1359 publications with VEGF and Retinal Diseases
Macular Degeneration Antibodies	>922 publications with VEGF and Macular Degeneration
Neoplasm Metastasis Antibodies	>481 publications with VEGF and Neoplasm Metastasis
Choroidal Neovascularization Antibodies	>463 publications with VEGF and Choroidal Neovascularization
Inflammation Antibodies	>440 publications with VEGF and Inflammation
Diabetic Retinopathy Antibodies	>433 publications with VEGF and Diabetic Retinopathy
Lung Diseases Antibodies	>394 publications with VEGF and Lung Diseases
Macular Edema Antibodies	>321 publications with VEGF and Macular Edema
Heart Diseases Antibodies	>268 publications with VEGF and Heart Diseases

Organs/Tissues associated with VEGF active protein

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>7635 publications with VEGF and Vascular
Blood Antibodies	>2914 publications with VEGF and Blood
Eye Antibodies	>1333 publications with VEGF and Eye
Lung Antibodies	>512 publications with VEGF and Lung
Bone Antibodies	>508 publications with VEGF and Bone
Muscle Antibodies	>447 publications with VEGF and Muscle
Embryonic Tissue Antibodies	>403 publications with VEGF and Embryonic Tissue
Heart Antibodies	>359 publications with VEGF and Heart
Liver Antibodies	>341 publications with VEGF and Liver
Kidney Antibodies	>324 publications with VEGF and Kidney