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Growth Hormone Mutant G119R (zfGH) Zebrafish Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400075

Recombinant zebrafish growth hormone mutant G113R (zbGH mutant) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 185 amino acids with an additional Ala at its N-terminus and having a molecular mass of 21187 Dalton. zfGH is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
growth hormone mutant G119R (zfGH), Active Protein
Full Product Name
Zebrafish growth hormone mutant G119R
Product Gene Name
zfGH active protein
Product Synonym Names
Zebrafish growth hormone mutant G113R (zfGH)
Sequence
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Gln-Ar g-Leu-Phe- Asn.
Host
E Coli
Form/Format
White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC.
Preparation and Storage
Lyophilized zfGH although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 mg/ml and filter sterilization zfGH mutant can be stored at 4 degree C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recomended. Please prevent freeze-thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized zfGH mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.42 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the DNAman program.
Dimers and Aggregates
The purified zfGH mutant (21K) consists of > 99% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.05 ng/ug (IEU/ug) of zfGH (22 K).
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.5% NaHCO3 adjusted to pH 8.
Biological Activity
The biologically activity of zfGH mutant in PDF-P1 3B9 cells stably transfected with rabbit GH receptors has not yet been tested.

Product Categories/Family for zfGH active protein

Growth Hormones; prolactins; placental lactogens

Other Notes

Small volumes of zfGH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for zfGH purchase

MBS400075 is an active protein targeting zfGH. Also known as Zebrafish growth hormone mutant G119R. Host: E Coli.
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