Mouse SMLA Active Protein – Cat# MBS400086

Super Leptin Antagonist (SMLA) Mouse Active Protein

Name: SMLA Active Protein; Super Leptin Antagonist; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS400086
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400086

Recombinant mouse leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, mLEP was mutated, resulting in D23L/L39A/D40A/F41A mutant that was purified by proprietary chromatographic… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

super leptin antagonist (SMLA), Active Protein

Full Product Name

Mouse super leptin antagonist (mutant D23/L39A/D40A/F41A)

Product Gene Name

SMLA active protein

Product Synonym Names

Mouse super-active leptin antagonist (SMLA)

Sequence

The sequence of the first five N-terminal amino acids of mouse super-acti ve leptin antagonist was determined and was found to be Ala-Val-Pr o-Ile-Gln

Host

Escherichia Coli

Form/Format

The mouse super-active leptin antagonist was lyophilized from a concetrated (1mg/ml) solution with 0.003mM NaHCO3.

Purity/Purification

The purity of mouse super-active leptin antagonist is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.

Preparation and Storage

Lyophilized mouse super-active leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -20 degree C.
Upon reconstitution of mouse super-active leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization mouse super-active leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized mouse super-active leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.

Protein Content

Protein quantization of mouse super-active leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.200 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

Dimers and Aggregates

The purified mouse super-active leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.

Endotoxin

The endotoxin content of mouse super-active leptin antagonist is less than 0.02 ng/ug (0.2 EU/ug) of mouse leptin antagonist.

Physical Appearance

White lyophilized (freeze-dried) powder.

Biological Activity

Mouse super-active leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Mouse super-active leptin antagonist also inhibits various leptin effects in several in vitro bioassays. The inhibitory activity of mouse super-active leptin antagonist was increased 14 to 60 fold as measured by various criteria such as binding properties to human leptin binding domain (hLBD) and in vitro and in vivo bioassays as compared to mouse leptin antagonist.

Usage

Mouse super-active leptin antagonist is furnished for laboratory research use only. The product may not be used as drugs, agriculutre or pesticidal products, food additives, local application, or household chemicals. It is for research purposes only- commercial use has been liscensed exclusivley to a third party.

Product Categories/Family for SMLA active protein

Leptin and leptin muteins

Other Notes

Small volumes of SMLA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMLA purchase

MBS400086 is an active protein targeting SMLA. Also known as Mouse super leptin antagonist (mutant D23/L39A/D40A/F41A). Host: Escherichia Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SMLA. It may not necessarily be applicable to this product.

Protein Family

Small aggregate formation protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.