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Leptin (lep) Salamander Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400096

Recombinant salamander leptin (Sal-LEP)is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kD. Recombinant salamander leptin was purified by proprietary chromatographic techniques,… More details

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Technical Datasheet View PDF
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Product Overview

Product Name
leptin, Active Protein
Full Product Name
Salamander leptin
Product Gene Name
Sequence
The sequence of the first ten N-terminal amino acids of recombinan t salamander leptin was determined and was found to be Ala-Ile-Me t-Val-Asp- Gln-Leu-Ar g-Met-Asp.
Host
E Coli
Form/Format
White lyophilized (freeze-dried) powder.
Purity/Purification
The purity of recombinant salamander leptin is greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) RP-chromatography
Preparation and Storage
Lyophilized recombinant salamander leptin although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of recombinant salamander leptin at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization recombinant salamander leptin can be stored at +4 degree C for at least two weeks.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized recombinant salamander leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100ug/ml. Recombinant salamander leptin, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Protein Content
Protein quantitation of recombinant salamander leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.104 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified Sal-LEP (16K) consists of > 99% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.05 ng/ug (IEU/ug) of recombinant salamander leptin
Formulation
The recombinant salamander leptin was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Biological Activity
recombinant salamander leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The activity of recombinant salamander leptin in that test is similar to that of mouse leptin (see Salomon et al (2006) Protein Expression and Purifcation 47, 128 -136).

Product Categories/Family for lep active protein

Other Notes

Small volumes of lep active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for lep purchase

MBS400096 is an active protein targeting lep. Also known as Salamander leptin. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for lep. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin<
NCBI Official Symbol
lep
NCBI Official Synonym Symbols
ob; xlep
NCBI Protein Information
leptin; obesity homolog

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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