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Leptin N82K Mutant Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400103

Recombinant protein - human leptin N82K mutant was produced by site specific mutagenesis. It consists of one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa., Human leptin mutant was… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
leptin N82K mutant, Active Protein
Full Product Name
Human leptin N82K mutant
Product Synonym Names
Human leptin N82K mutant LEP-15
Sequence
The sequence of the first five N-terminal amino acids of recombinan t human leptin N82K mutant was determined and was found to be Ala-Val-Pr o-Ile-Gln.
Host
Escherichia Coli
Form/Format
White lyophilized (freeze-dried) powder.
Purity/Purification
Purity of recombinant human leptin N82K mutant is greater than 98.0% as determined by:
(a) Gel filtration analysis of the recombinant protein.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Preparation and Storage
Lyophilized recombinant human leptin N82K mutant although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 mg human leptin per ml and up to 2 mg and filter sterilization recombinant human leptin N82K mutant can be stored at +4 degree C or even room temperature for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized recombinant human leptin N82K mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation in recombinant human leptin N82K mutant was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.87 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified recombinant human leptin (16K) N82K mutant consists of > 98% monomers as determined by gel-filtration chromatography.
Endotoxin
The endotoxin content of recombinant human leptin is less than 0.05 ng/ug (0.5 IEU/ug).
Formulation
The recombinant protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Biological Activity
recombinant human leptin N82K mutant is less than 0.1% active active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. This abolishment of activity results from drastically reduced affinity toward leptin receptor.

Product Categories/Family for leptin N82K mutant active protein

Leptin and leptin muteins

Other Notes

Small volumes of leptin N82K mutant active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for leptin N82K mutant purchase

MBS400103 is an active protein targeting leptin N82K mutant. Also known as Human leptin N82K mutant. Host: Escherichia Coli.
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