Pegylated Leptin N82K Mutant Human Active Protein

Name: Active Protein; Pegylated Leptin N82K Mutant; Human; MyBioSource
Brand: MyBioSource
SKU: MBS400106
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS400106

Mono-pegylated (with 20 kDa PEG) recombinant human leptin N82K mutant is an one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

pegylated leptin N82K mutant, Active Protein

Full Product Name

Human pegylated leptin N82K mutant

Product Synonym Names

Pegylated human leptin N82K mutant

Sequence

The sequence of the N-terminal amino acids of recombinan t human pegylated leptin could not be determined indicating that the PEG moiety is attached to the N-terminus .

Host

E Coli

Form/Format

The recombinant human pegylated leptin N82K mutant was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3 and the powder contains 35 to 40% protein.
Physical Appearance: White lyophilized (freeze-dried) powder.

Purity/Purification

The purity od recombinant human pegylated leptin N82K mutant is greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.

Preparation and Storage

Lyophilized recombinant human pegylated leptin N82K mutant although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 recombinant human pegylated leptin mg/ml and up to 2 mg/ml and filter sterilization recombinant human pegylated leptin can be stored at 4C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of recombinant human pegylated leptin N82K mutant addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Dimers and Aggregates

The purified recombinant human pegylated leptin consists of > 95% monomers as determined by gel-filtration chromatography.

Biological Activity

MyBioSource's recombinant pegylated human leptin N82K mutant is less than 0.1% active active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. This abolishment of activity results from drastically reduced affinity toward leptin receptor.

Endotoxin

Less than 0.05 ng/ug (0.5 IEU/ug) of recombinant human pegylated leptin N82K mutant..

Protein Content

Protein quantitation of recombinant human pegylated leptin N82K mutant was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.870 as the extinction coefficient for a 0.1% (1mg/ml) protein solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

Solubility

It is recommended to reconstitute the lyophilized recombinant human pegylated leptin N82K mutant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.

Product Categories/Family for pegylated leptin N82K mutant active protein

Other Notes

Small volumes of pegylated leptin N82K mutant active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for pegylated leptin N82K mutant purchase

MBS400106 is an active protein targeting pegylated leptin N82K mutant. Also known as Human pegylated leptin N82K mutant. Host: E Coli.

Pegylated Leptin N82K Mutant Human Active Protein

Product Overview

Product Categories/Family for pegylated leptin N82K mutant active protein

Related Product Information for pegylated leptin N82K mutant active protein

Other Notes

Searchable Terms for pegylated leptin N82K mutant purchase